pUC-TalO8 (Blank-CoTrIP)
(Plasmid
#178303)
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Purpose(Empty Backbone) Blank CoTrIP plasmid
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Depositing Lab
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Sequence Information
Ordering
| Item | Catalog # | Description | Quantity | Price (USD) | |
|---|---|---|---|---|---|
| Plasmid | 178303 | Standard format: Plasmid sent in bacteria as agar stab | 1 | $85 | |
This material is available to academics and nonprofits only.
Backbone
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Vector backbonepUC
- Backbone size (bp) 2700
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Modifications to backboneInsertion of Trp1-ARS-8xLacO
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Vector typeYeast Expression
- Promoter NA
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Selectable markersTRP1
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Tag
/ Fusion Protein
- NA
Growth in Bacteria
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Bacterial Resistance(s)Ampicillin, 100 μg/mL
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Growth Temperature37°C
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Growth Strain(s)DH5alpha
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Copy numberUnknown
Cloning Information
- Cloning method Unknown
- 5′ sequencing primer M13
- 3′ sequencing primer M13 (Common Sequencing Primers)
Resource Information
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Supplemental Documents
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A portion of this plasmid was derived from a plasmid made byToshi Tsukiyama. Unnikrishnan et al. Nat Struct Mol Biol. 2010 Apr;17(4):430-7. doi: 10.1038/nsmb.1780. Epub 2010 Mar 14.
Terms and Licenses
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Academic/Nonprofit Terms
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Industry Terms
- Not Available to Industry
Trademarks:
- Zeocin® is an InvivoGen trademark.
Depositor Comments
Please visit https://doi.org/10.1101/2021.10.17.464753 for bioRxiv preprint.
How to cite this plasmid
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These plasmids were created by your colleagues. Please acknowledge the Principal Investigator, cite the article in which the plasmids were described, and include Addgene in the Materials and Methods of your future publications.
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For your Materials & Methods section:
pUC-TalO8 (Blank-CoTrIP) was a gift from Brian Zid (Addgene plasmid # 178303 ; http://n2t.net/addgene:178303 ; RRID:Addgene_178303) -
For your References section:
Rvb1/Rvb2 proteins couple transcription and translation during glucose starvation. Chen YS, Hou W, Tracy S, Harvey AT, Harjono V, Xu F, Moresco JJ, Yates JR 3rd, Zid BM. Elife. 2022 Sep 15;11. pii: 76965. doi: 10.7554/eLife.76965. 10.7554/eLife.76965 PubMed 36107469
