pETM33_MDM2_SWIB
(Plasmid #178481)

• Purpose: Bacterial expression of human domain with His-tag and GST tag
• Depositing Lab: Ylva Ivarsson
• Publication: Benz et al Mol Syst Biol. 2022 Jan;18(1):e10584. doi: 10.15252/msb.202110584.

Backbone

• Vector backbone: pETM33
• Backbone size w/o insert (bp): 6017
• Vector type: Bacterial Expression

Growth in Bacteria

• Bacterial Resistance(s): Kanamycin, 50 μg/mL
• Growth Temperature: 37°C
• Growth Strain(s): DH5alpha
• Copy number: Unknown

Gene/Insert

• Gene/Insert name: MDM2_SWIB
• Species: H. sapiens (human); Synthetic
• Entrez Gene: MDM2 (a.k.a. ACTFS, HDMX, LSKB, hdm2)
• Promoter: T7/LacO
• Tag / Fusion Protein:
  • His-GST (N terminal on insert)

Cloning Information

• Cloning method: Restriction Enzyme
• 5′ cloning site: Nco1 (destroyed during cloning)
• 3′ cloning site: EcoR1 (destroyed during cloning)
• 5′ sequencing primer: CGGATCTGGAAGTTCTGTTCC
• 3′ sequencing primer: GAGTGCGGCCGCAAGCTTG

Terms and Licenses

• Academic/Nonprofit Terms:
  • UBMTA
• Industry Terms:
  • Not Available to Industry

Depositor Comments

Please visit https://www.biorxiv.org/content/10.1101/2021.04.13.439572v1 for bioRxv preprint. Growth in BL21 DE3 gold 37 C after induction with 1mM IPTG 4 hours 30 C. Insert: mgSQIPASEQETLVRPKPLLLKLLKSVGAQKDTYTMKEVLFYLGQYIMTKRLYDEKQQHIVYCSNDLLGDLFGVPSFSVKEHRKIYTMIYRNLVVVNQQESSDSGTSVSEN.