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(Plasmid #17860)


Item Catalog # Description Quantity Price (USD)
Plasmid 17860 Standard format: Plasmid sent in bacteria as agar stab 1 $85

This material is available to academics and nonprofits only.


  • Vector backbone
  • Backbone manufacturer
  • Backbone size w/o insert (bp) 2900
  • Vector type
    Bacterial Expression

Growth in Bacteria

  • Bacterial Resistance(s)
    Ampicillin, 100 μg/mL
  • Growth Temperature
  • Growth Strain(s)
  • Copy number
    High Copy


  • Gene/Insert name
  • Alt name
  • Alt name
  • Alt name
    Ribose Binding Protein
  • Species
    E. coli
  • Insert Size (bp)
  • Mutation
  • Tags / Fusion Proteins
    • ECFP (N terminal on insert)
    • EYFP (C terminal on insert)

Cloning Information

Terms and Licenses

  • Academic/Nonprofit Terms
  • Industry Terms
    • Not Available to Industry
  • Zeocin® is an InvivoGen trademark.

Depositor Comments

A RBP PCR product from genomic E. coli DNA encoding mature RBP was cloned into the KpnI site of FLIPglu-600u in pRSETB, replacing the glucose sensor's mglB moiety. The RBP sequence was found to carry a D208G substitution, which does not significantly affect the binding affinity.

How to cite this plasmid ( Back to top)

These plasmids were created by your colleagues. Please acknowledge the Principal Investigator, cite the article in which the plasmids were described, and include Addgene in the Materials and Methods of your future publications.

  • For your Materials & Methods section:

    FLIPrbs was a gift from Wolf Frommer (Addgene plasmid # 17860 ; ; RRID:Addgene_17860)
  • For your References section:

    Development of a fluorescent nanosensor for ribose. Lager I, Fehr M, Frommer WB, Lalonde S. FEBS Lett. 2003 Oct 9. 553(1-2):85-9. 10.1016/S0014-5793(03)00976-1 PubMed 14550551