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pmcherry α-catenin L344P
(Plasmid #178647)

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This material is available to academics and nonprofits only.
Item Catalog # Description Quantity Price (USD)
Plasmid 178647 Standard format: Plasmid sent in bacteria as agar stab 1 $94
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DNA 178647-D50 50 μg of DNA in Tris buffer $495
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DNA 178647-D100 100 μg of DNA in Tris buffer $585
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Backbone

  • Vector backbone
    pmcherryC1
  • Backbone size w/o insert (bp) 4721
  • Total vector size (bp) 7400
  • Vector type
    Mammalian Expression
  • Selectable markers
    Neomycin (select with G418)

Growth in Bacteria

  • Bacterial Resistance(s)
    Kanamycin, 50 μg/mL
  • Growth Temperature
    37°C
  • Growth Strain(s)
    DH5alpha
  • Copy number
    High Copy

Gene/Insert

  • Gene/Insert name
    α-catenin
  • Alt name
    CTNNA1
  • Species
    H. sapiens (human)
  • Mutation
    L344P
  • GenBank ID
    NM_001903
  • Entrez Gene
    CTNNA1 (a.k.a. CAP102, MDBS2, MDPT2)
  • Tag / Fusion Protein
    • mcherry (N terminal on insert)

Cloning Information

Resource Information

  • A portion of this plasmid was derived from a plasmid made by
    insert with L344P mutation was obtained from Kris DeMali (Peng, JBC 2012)

Terms and Licenses

  • Academic/Nonprofit Terms
  • Industry Terms
    • Not Available to Industry

Trademarks:

  • Zeocin® is an InvivoGen trademark.

Purpose

Ready-to-use, high-purity DNA prep. DNA aliquots are suitable for use in mammalian cell transfections and other molecular biology applications.

Delivery

  • Amount 50 μg
  • Concentration 1 μg/μL
  • Pricing $495 USD
  • Storage 4 ℃ (short-term) or -20 ℃ (long-term)

Terms and Licenses

  • Academic/Nonprofit Terms
  • Industry Terms
    • Not Available to Industry

Quality Control

Every DNA prep is verified by next-generation sequencing, confirming plasmid identity, sequence integrity, and absence of DNA contaminants. Concentration is confirmed to be within ±2.5% of the stated concentration.

Our production process has been validated to consistently yield endotoxin levels below 1.0 EU/μg. Individual lots are not tested for endotoxin.

Purpose

Ready-to-use, high-purity DNA prep. DNA aliquots are suitable for use in mammalian cell transfections and other molecular biology applications.

Delivery

  • Amount 100 μg
  • Concentration 1 μg/μL
  • Pricing $585 USD
  • Storage 4 ℃ (short-term) or -20 ℃ (long-term)

Terms and Licenses

  • Academic/Nonprofit Terms
  • Industry Terms
    • Not Available to Industry

Quality Control

Every DNA prep is verified by next-generation sequencing, confirming plasmid identity, sequence integrity, and absence of DNA contaminants. Concentration is confirmed to be within ±2.5% of the stated concentration.

Our production process has been validated to consistently yield endotoxin levels below 1.0 EU/μg. Individual lots are not tested for endotoxin.

How to cite this plasmid ( Back to top)

These plasmids were created by your colleagues. Please acknowledge the Principal Investigator, cite the article in which the plasmids were described, and include Addgene in the Materials and Methods of your future publications.

  • For your Materials & Methods section:

    pmcherry α-catenin L344P was a gift from Daniel Conway (Addgene plasmid # 178647 ; http://n2t.net/addgene:178647 ; RRID:Addgene_178647)

  • For your References section:

    alpha-catenin Dependent Vinculin Recruitment to Adherens Junctions is Antagonistic to Focal Adhesions. Bejar-Padilla V, Cabe JI, Lopez S, Narayanan V, Mezher M, Maruthamuthu V, Conway DE. Mol Biol Cell. 2022 Aug 3:mbcE22020071. doi: 10.1091/mbc.E22-02-0071. 10.1091/mbc.E22-02-0071 PubMed 35921161
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