pCMV-FKBP-mCherry-MCS
(Plasmid
#178863)
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PurposeMammalian expression of a protein of interest fused to the C-terminus of FKBP-mCherry
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Depositing Lab
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Depositing OrganizationNagoya Institute of Technology
Located in Japan -
Sequence Information
Ordering
| Item | Catalog # | Description | Quantity | Price (USD) | |
|---|---|---|---|---|---|
| Plasmid | 178863 | Standard format: Plasmid sent in bacteria as agar stab | 1 | $94 | |
| DNA | 178863-D50 | 50 μg of DNA in Tris buffer | $495 | ||
| DNA | 178863-D100 | 100 μg of DNA in Tris buffer | $585 | ||
Backbone
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Vector backbonepmCherry-C1
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Backbone manufacturerClontech
- Backbone size w/o insert (bp) 4713
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Vector typeMammalian Expression
Growth in Bacteria
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Bacterial Resistance(s)Kanamycin, 50 μg/mL
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Growth Temperature37°C
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Growth Strain(s)DH5alpha
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Copy numberHigh Copy
Gene/Insert
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Gene/Insert nameFKBP-mCherry
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SpeciesSynthetic
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Insert Size (bp)354
- Promoter CMV
Cloning Information
- Cloning method Restriction Enzyme
- 5′ cloning site NheI (not destroyed)
- 3′ cloning site AgeI (not destroyed)
Resource Information
Terms and Licenses
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Academic/Nonprofit Terms
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Industry Terms
- Not Available to Industry
Trademarks:
- Zeocin® is an InvivoGen trademark.
DNA (Catalog # 178863-D50)
Purpose
Ready-to-use, high-purity DNA prep. DNA aliquots are suitable for use in mammalian cell transfections and other molecular biology applications.
Delivery
- Amount 50 μg
- Concentration 1 μg/μL
- Pricing $495 USD
- Storage 4 ℃ (short-term) or -20 ℃ (long-term)
Terms and Licenses
-
Academic/Nonprofit Terms
-
Industry Terms
- Not Available to Industry
Quality Control
Every DNA prep is verified by next-generation sequencing, confirming plasmid identity, sequence integrity, and absence of DNA contaminants. Concentration is confirmed to be within ±2.5% of the stated concentration.
Our production process has been validated to consistently yield endotoxin levels below 1.0 EU/μg. Individual lots are not tested for endotoxin.
DNA (Catalog # 178863-D100)
Purpose
Ready-to-use, high-purity DNA prep. DNA aliquots are suitable for use in mammalian cell transfections and other molecular biology applications.
Delivery
- Amount 100 μg
- Concentration 1 μg/μL
- Pricing $585 USD
- Storage 4 ℃ (short-term) or -20 ℃ (long-term)
Terms and Licenses
-
Academic/Nonprofit Terms
-
Industry Terms
- Not Available to Industry
Quality Control
Every DNA prep is verified by next-generation sequencing, confirming plasmid identity, sequence integrity, and absence of DNA contaminants. Concentration is confirmed to be within ±2.5% of the stated concentration.
Our production process has been validated to consistently yield endotoxin levels below 1.0 EU/μg. Individual lots are not tested for endotoxin.
These plasmids were created by your colleagues. Please acknowledge the Principal Investigator, cite the article in which the plasmids were described, and include Addgene in the Materials and Methods of your future publications.
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For your Materials & Methods section:
pCMV-FKBP-mCherry-MCS was a gift from Shinya Tsukiji (Addgene plasmid # 178863 ; http://n2t.net/addgene:178863 ; RRID:Addgene_178863) -
For your References section:
Synthetic Protein Condensates That Inducibly Recruit and Release Protein Activity in Living Cells. Yoshikawa M, Yoshii T, Ikuta M, Tsukiji S. J Am Chem Soc. 2021 May 5;143(17):6434-6446. doi: 10.1021/jacs.0c12375. Epub 2021 Apr 23. 10.1021/jacs.0c12375 PubMed 33890764
