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Addgene

pCMV-PB1-AG-FRB-TEVcs-mTagBFP2-HA-Vav2cat
(Plasmid #178870)

Ordering

This material is available to academics and nonprofits only.
Item Catalog # Description Quantity Price (USD)
Plasmid 178870 Standard format: Plasmid sent in bacteria as agar stab 1 $85

Backbone

  • Vector backbone
    pAsh-MCL
  • Backbone manufacturer
    MBL International
  • Backbone size w/o insert (bp) 4244
  • Vector type
    Mammalian Expression

Growth in Bacteria

  • Bacterial Resistance(s)
    Kanamycin, 50 μg/mL
  • Growth Temperature
    37°C
  • Growth Strain(s)
    DH5alpha
  • Copy number
    High Copy

Gene/Insert

  • Gene/Insert name
    PB1-AzamiGreen-FRB-TEVcs(ENLYFQL)-mTagBFP2-HA-Vav2(DH-PH-CRD)
  • Species
    M. musculus (mouse)
  • Insert Size (bp)
    3412
  • Entrez Gene
    Vav2 (a.k.a. 2810040F13Rik, AI847175, Vav-2)
  • Promoter CMV

Cloning Information

  • Cloning method Restriction Enzyme
  • 5′ cloning site AgeI (not destroyed)
  • 3′ cloning site NotI (not destroyed)

Resource Information

Terms and Licenses

Trademarks:
  • Zeocin® is an InvivoGen trademark.
How to cite this plasmid ( Back to top)

These plasmids were created by your colleagues. Please acknowledge the Principal Investigator, cite the article in which the plasmids were described, and include Addgene in the Materials and Methods of your future publications.

  • For your Materials & Methods section:

    pCMV-PB1-AG-FRB-TEVcs-mTagBFP2-HA-Vav2cat was a gift from Shinya Tsukiji (Addgene plasmid # 178870 ; http://n2t.net/addgene:178870 ; RRID:Addgene_178870)
  • For your References section:

    Synthetic Protein Condensates That Inducibly Recruit and Release Protein Activity in Living Cells. Yoshikawa M, Yoshii T, Ikuta M, Tsukiji S. J Am Chem Soc. 2021 May 5;143(17):6434-6446. doi: 10.1021/jacs.0c12375. Epub 2021 Apr 23. 10.1021/jacs.0c12375 PubMed 33890764