pCAGGS-PB1-AG(Y63L)-LOV2
(Plasmid
#178876)
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PurposeMammalian expression of PB1-AzamiGreen(Y63L)-LOV2
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Depositing Lab
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Depositing OrganizationNagoya Institute of Technology
Located in Japan -
Sequence Information
Ordering
| Item | Catalog # | Description | Quantity | Price (USD) | |
|---|---|---|---|---|---|
| Plasmid | 178876 | Standard format: Plasmid sent in bacteria as agar stab | 1 | $94 | |
| DNA | 178876-D50 | 50 μg of DNA in Tris buffer | $495 | ||
| DNA | 178876-D100 | 100 μg of DNA in Tris buffer | $585 | ||
Backbone
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Vector backbonepCAGGS
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Backbone manufacturerDr. Jun-ichi Miyazaki (Osaka University, Japan)
- Backbone size w/o insert (bp) 4091
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Vector typeMammalian Expression
Growth in Bacteria
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Bacterial Resistance(s)Ampicillin, 100 μg/mL
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Growth Temperature37°C
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Growth Strain(s)DH5alpha
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Copy numberHigh Copy
Gene/Insert
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Gene/Insert namePB1-AzamiGreen(Y63L)-LOV2
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SpeciesSynthetic
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Insert Size (bp)1864
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MutationAzamiGreen: Y63L
- Promoter CAG
Cloning Information
- Cloning method Restriction Enzyme
- 5′ cloning site EcoRI (not destroyed)
- 3′ cloning site NotI (not destroyed)
Resource Information
Terms and Licenses
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Academic/Nonprofit Terms
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Industry Terms
- Not Available to Industry
Trademarks:
- Zeocin® is an InvivoGen trademark.
DNA (Catalog # 178876-D50)
Purpose
Ready-to-use, high-purity DNA prep. DNA aliquots are suitable for use in mammalian cell transfections and other molecular biology applications.
Delivery
- Amount 50 μg
- Concentration 1 μg/μL
- Pricing $495 USD
- Storage 4 ℃ (short-term) or -20 ℃ (long-term)
Terms and Licenses
-
Academic/Nonprofit Terms
-
Industry Terms
- Not Available to Industry
Quality Control
Every DNA prep is verified by next-generation sequencing, confirming plasmid identity, sequence integrity, and absence of DNA contaminants. Concentration is confirmed to be within ±2.5% of the stated concentration.
Our production process has been validated to consistently yield endotoxin levels below 1.0 EU/μg. Individual lots are not tested for endotoxin.
DNA (Catalog # 178876-D100)
Purpose
Ready-to-use, high-purity DNA prep. DNA aliquots are suitable for use in mammalian cell transfections and other molecular biology applications.
Delivery
- Amount 100 μg
- Concentration 1 μg/μL
- Pricing $585 USD
- Storage 4 ℃ (short-term) or -20 ℃ (long-term)
Terms and Licenses
-
Academic/Nonprofit Terms
-
Industry Terms
- Not Available to Industry
Quality Control
Every DNA prep is verified by next-generation sequencing, confirming plasmid identity, sequence integrity, and absence of DNA contaminants. Concentration is confirmed to be within ±2.5% of the stated concentration.
Our production process has been validated to consistently yield endotoxin levels below 1.0 EU/μg. Individual lots are not tested for endotoxin.
These plasmids were created by your colleagues. Please acknowledge the Principal Investigator, cite the article in which the plasmids were described, and include Addgene in the Materials and Methods of your future publications.
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For your Materials & Methods section:
pCAGGS-PB1-AG(Y63L)-LOV2 was a gift from Shinya Tsukiji (Addgene plasmid # 178876 ; http://n2t.net/addgene:178876 ; RRID:Addgene_178876) -
For your References section:
Synthetic Protein Condensates That Inducibly Recruit and Release Protein Activity in Living Cells. Yoshikawa M, Yoshii T, Ikuta M, Tsukiji S. J Am Chem Soc. 2021 May 5;143(17):6434-6446. doi: 10.1021/jacs.0c12375. Epub 2021 Apr 23. 10.1021/jacs.0c12375 PubMed 33890764
