pBBK47 pHomL-RPL18Bp-SRM1-Link-Neon(gfp)-SSA1t-HomR (AmpR)
(Plasmid #179031)

• Purpose: Vector containing a yeast integration cassette for RPL18B-driven expression of SRM1-Link-Neon(gfp), a marker for the nucleus
• Depositing Lab: Vincent Martin
• Publication: Bean et al G3 (Bethesda). 2022 Jun 16. pii: 6609175. doi: 10.1093/g3journal/jkac154.

Backbone

• Vector backbone: pYTK089 (ColE1, AmpR)
• Vector type: Yeast Expression

Growth in Bacteria

• Bacterial Resistance(s): Ampicillin, 100 μg/mL
• Growth Temperature: 37°C
• Growth Strain(s): DH5alpha
• Copy number: High Copy

Gene/Insert

• Gene/Insert name: RPL18Bp-SRM1-Link-Neon(gfp)
• Species: S. cerevisiae (budding yeast)

Cloning Information

• Cloning method: Restriction Enzyme
• 5′ cloning site: Unknown (unknown if destroyed)
• 3′ cloning site: Unknown (unknown if destroyed)

Resource Information

• Supplemental Documents:
  • pbbk47-phoml-rpl18bp-srm1-link-neongfp-ssa1t-homr-ampr.gb

Terms and Licenses

• Academic/Nonprofit Terms:
  • UBMTA
  • mNeonGreen Label License
• Industry Terms:
  • Not Available to Industry

Depositor Comments

Part of the Markerless Yeast Localization and Overexpression (MyLO) CRISPR-Cas9 Toolkit. Vector cloned using BsaI Golden Gate cloning