Flag-mCherry-Binder
(Plasmid #179139)

• Purpose: Binder: SspB that carries two point mutations reducing homodimerization(Y11K/A15E, residue numbering from pdb: 1ou9). Binder binds to the 7 a.a. peptide SsrA (AANDENY).
• Depositing Lab: Klaus Hahn
• Publication: Liu et al Cell. 2021 Oct 28;184(22):5670-5685.e23. doi: 10.1016/j.cell.2021.09.026. Epub 2021 Oct 11.

Backbone

• Vector backbone: pTriEx
• Backbone size w/o insert (bp): 5000
• Total vector size (bp): 6105
• Vector type: Mammalian Expression

Growth in Bacteria

• Bacterial Resistance(s): Ampicillin, 100 μg/mL
• Growth Temperature: 37°C
• Growth Strain(s): DH5alpha
• Copy number: High Copy

Gene/Insert

• Gene/Insert name: SspB
• Alt name: Binder
• Species: Haemophilus influenzae
• Insert Size (bp): 1092
• Mutation: Y11K/A15E, residue numbering from pdb: 1ou9
• Tags / Fusion Proteins:
  • mCherry (N terminal on insert)
  • FLAG (N terminal on insert)

Resource Information

• Supplemental Documents:
  • Flag-mCherry-Binder.gb

Terms and Licenses

• Academic/Nonprofit Terms:
  • UBMTA
  • Takara Bio Limited Use Label License (formerly Clontech)
• Industry Terms:
  • Not Available to Industry