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pMC0x_gRNA_Pos4_Ptet
(Plasmid #179335)

Ordering

Item Catalog # Description Quantity Price (USD)
Plasmid 179335 Standard format: Plasmid sent in bacteria as agar stab 1 $85

This material is available to academics and nonprofits only.

Backbone

  • Vector backbone
    pMC_V*_01_LVL0* Entry sfGFP (Marburg Collection)
  • Vector type
    CRISPR

Growth in Bacteria

  • Bacterial Resistance(s)
    Kanamycin, 50 μg/mL
  • Growth Temperature
    37°C
  • Growth Strain(s)
    DH5alpha
  • Copy number
    High Copy

Gene/Insert

  • Gene/Insert name
    Ptet gRNA with sfGFP dropout
  • Species
    A. victoria

Cloning Information

  • Cloning method Restriction Enzyme
  • 5′ cloning site unknown (unknown if destroyed)

Resource Information

Terms and Licenses

  • Academic/Nonprofit Terms
  • Industry Terms
    • Not Available to Industry
Trademarks:
  • Zeocin® is an InvivoGen trademark.

Depositor Comments

How to cite this plasmid ( Back to top)

These plasmids were created by your colleagues. Please acknowledge the Principal Investigator, cite the article in which the plasmids were described, and include Addgene in the Materials and Methods of your future publications.

  • For your Materials & Methods section:

    pMC0x_gRNA_Pos4_Ptet was a gift from Anke Becker (Addgene plasmid # 179335 ; http://n2t.net/addgene:179335 ; RRID:Addgene_179335)
  • For your References section:

    NT-CRISPR, combining natural transformation and CRISPR-Cas9 counterselection for markerless and scarless genome editing in Vibrio natriegens. Stukenberg D, Hoff J, Faber A, Becker A. Commun Biol. 2022 Mar 25;5(1):265. doi: 10.1038/s42003-022-03150-0. 10.1038/s42003-022-03150-0 PubMed 35338236