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pET21a Hp-iPGM-bio
(Plasmid #179615)


Item Catalog # Description Quantity Price (USD)
Plasmid 179615 Standard format: Plasmid sent in bacteria as agar stab 1 $85

This material is available to academics and nonprofits only.


  • Vector backbone
  • Backbone manufacturer
    EMD Biosciences
  • Backbone size w/o insert (bp) 5443
  • Total vector size (bp) 6957
  • Modifications to backbone
    1. Bm-iPGM-AviTag construct in pET21a was modified using site directed mutagenesis to remove the Xho restriction site located after AviTag and generate a universal backbone containing Avitag for easy cloning in frame with His tag. The resulting construct is as follows: BamH1 – Bm-iPGM– Xho1-AviTag-no Xho1-6xHis-stop 2. Bm-iPGM-AviTag is removed using restriction enzymes BamH1/Xho1 and Hp-iPGM (synthetized by BioBasic) is cloned into BamH1/Xho1 restriction sites. The resulting construct is as follows: BamH1 – Hp-iPGM– Xho1-AviTag-no Xho1-6xHis-stop
  • Vector type
    Bacterial Expression

Growth in Bacteria

  • Bacterial Resistance(s)
    Ampicillin, 100 μg/mL
  • Growth Temperature
  • Growth Strain(s)
  • Copy number
    High Copy


  • Gene/Insert name
    H. pylori iPGM bio-6His
  • Alt name
    Hp iPGM bio
  • Alt name
    2,3-bisphosphoglycerate-independent phosphoglycerate mutase
  • Species
    Helicobacter pylori
  • Insert Size (bp)
  • GenBank ID
  • Promoter T7
  • Tags / Fusion Proteins
    • biotinylation sequence (C terminal on backbone)
    • His tag (C terminal on backbone)

Cloning Information

  • Cloning method Restriction Enzyme
  • 5′ cloning site BamH1 (not destroyed)
  • 3′ cloning site Xho1 (not destroyed)
  • 5′ sequencing primer T7 promotor primer #69348-3
  • 3′ sequencing primer T7 terminator primer #69337-3
  • (Common Sequencing Primers)

Terms and Licenses

  • Academic/Nonprofit Terms
  • Industry Terms
    • Not Available to Industry
  • Zeocin® is an InvivoGen trademark.
How to cite this plasmid ( Back to top)

These plasmids were created by your colleagues. Please acknowledge the Principal Investigator, cite the article in which the plasmids were described, and include Addgene in the Materials and Methods of your future publications.

  • For your Materials & Methods section:

    pET21a Hp-iPGM-bio was a gift from James Inglese (Addgene plasmid # 179615 ; ; RRID:Addgene_179615)
  • For your References section:

    Serum-Stable and Selective Backbone-N-Methylated Cyclic Peptides That Inhibit Prokaryotic Glycolytic Mutases. van Neer RHP, Dranchak PK, Liu L, Aitha M, Queme B, Kimura H, Katoh T, Battaile KP, Lovell S, Inglese J, Suga H. ACS Chem Biol. 2022 Aug 19;17(8):2284-2295. doi: 10.1021/acschembio.2c00403. Epub 2022 Jul 29. 10.1021/acschembio.2c00403 PubMed 35904259