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CMV-HA-hTAF5
(Plasmid #17984)

Full plasmid sequence is not available for this item.

Ordering

Item Catalog # Description Quantity Price (USD)
Plasmid 17984 Standard format: Plasmid sent in bacteria as agar stab 1 $65

This material is available to academics and nonprofits only.

Backbone

Growth in Bacteria

  • Bacterial Resistance(s)
    Ampicillin
  • Growth Temperature
    37°C
  • Growth Strain(s)
    DH5alpha
  • Copy number
    Unknown

Gene/Insert

  • Gene/Insert name
    TAF5
  • Alt name
    TAF100
  • Alt name
    hTAFII100
  • Species
    H. sapiens (human)
  • Insert Size (bp)
    2500
  • GenBank ID
    U75309
  • Entrez Gene
    TAF5 (a.k.a. TAF(II)100, TAF2D, TAFII-100, TAFII100)
  • Tag / Fusion Protein
    • HA (N terminal on backbone)

Cloning Information

  • Cloning method Restriction Enzyme
  • 5′ cloning site Nde I (not destroyed)
  • 3′ cloning site Xho I (not destroyed)
  • 5′ sequencing primer CMV-F
  • (Common Sequencing Primers)

Resource Information

  • Terms and Licenses
How to cite this plasmid ( Back to top)

These plasmids were created by your colleagues. Please acknowledge the Principal Investigator, cite the article in which the plasmids were described, and include Addgene in the Materials and Methods of your future publications.

  • For your Materials & Methods section:

    CMV-HA-hTAF5 was a gift from Naoko Tanese (Addgene plasmid # 17984 ; http://n2t.net/addgene:17984 ; RRID:Addgene_17984)
  • For your References section:

    Molecular cloning and analysis of two subunits of the human TFIID complex: hTAFII130 and hTAFII100. Tanese N, Saluja D, Vassallo MF, Chen JL, Admon A. Proc Natl Acad Sci U S A. 1996 Nov 26. 93(24):13611-6. 10.1073/pnas.93.24.13611 PubMed 8942982