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pcDNA3.1 FLIPglu-30uDelta13V ECFP-W67G
(Plasmid #18017)


Item Catalog # Description Quantity Price (USD)
Plasmid 18017 Standard format: Plasmid sent in bacteria as agar stab 1 $85

This material is available to academics and nonprofits only.


  • Vector backbone
  • Backbone manufacturer
  • Backbone size w/o insert (bp) 5428
  • Vector type
    Mammalian Expression
  • Selectable markers
    Neomycin (select with G418)

Growth in Bacteria

  • Bacterial Resistance(s)
    Ampicillin, 100 μg/mL
  • Growth Temperature
  • Growth Strain(s)
  • Copy number
    High Copy


  • Gene/Insert name
    FLIPglu D154A Delta13V ECFP-W67G
  • Alt name
  • Alt name
    Periplasmic glucose binding
  • Species
    E. coli
  • Insert Size (bp)
  • Mutation
    mglB-D154A; non-functional ECFP
  • Tags / Fusion Proteins
    • ECFP-W67G (N terminal on insert)
    • Venus (C terminal on insert)

Cloning Information

  • Cloning method Restriction Enzyme
  • 5′ cloning site NcoI (not destroyed)
  • 3′ cloning site XbaI (not destroyed)
  • 5′ sequencing primer T7 Fwd
  • (Common Sequencing Primers)

Terms and Licenses

  • Academic/Nonprofit Terms
  • Industry Terms
    • Not Available to Industry
  • Zeocin® is an InvivoGen trademark.
How to cite this plasmid ( Back to top)

These plasmids were created by your colleagues. Please acknowledge the Principal Investigator, cite the article in which the plasmids were described, and include Addgene in the Materials and Methods of your future publications.

  • For your Materials & Methods section:

    pcDNA3.1 FLIPglu-30uDelta13V ECFP-W67G was a gift from Wolf Frommer (Addgene plasmid # 18017 ; ; RRID:Addgene_18017)
  • For your References section:

    Facilitative plasma membrane transporters function during ER transit. Takanaga H, Frommer WB. FASEB J. 2010 Aug . 24(8):2849-58. 10.1096/fj.09-146472 PubMed 20354141