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pTRIP TRE BI_SEPT9_i3-14-GFP10_GFP11-14-SEPT7 Gmut2
(Plasmid #180361)


Item Catalog # Description Quantity Price (USD)
Plasmid 180361 Standard format: Plasmid sent in bacteria as agar stab 1 $85

This material is available to academics and nonprofits only.


Growth in Bacteria

  • Bacterial Resistance(s)
    Ampicillin, 100 μg/mL
  • Growth Temperature
  • Growth Strain(s)
    NEB Stable
  • Copy number
    High Copy

Gene/Insert 1

  • Gene/Insert name
  • Alt name
  • Species
    H. sapiens (human)
  • Mutation
    SEPT7 H279D
  • Entrez Gene
    SEPTIN7 (a.k.a. CDC10, CDC3, NBLA02942, SEPT7, SEPT7A)
  • Promoter bidirectional TRE
  • Tag / Fusion Protein
    • GFP11 (N terminal on insert)

Cloning Information for Gene/Insert 1

Gene/Insert 2

  • Gene/Insert name
  • Alt name
  • Alt name
  • Species
    H. sapiens (human)
  • Entrez Gene
    SEPTIN9 (a.k.a. AF17q25, MSF, MSF1, NAPB, PNUTL4, SEPT9, SINT1, SeptD1)
  • Promoter bidirectional TRE
  • Tag / Fusion Protein
    • GFP10 (C terminal on insert)

Cloning Information for Gene/Insert 2

Gene/Insert 3

  • Gene/Insert name

Resource Information

Terms and Licenses

  • Zeocin® is an InvivoGen trademark.

Depositor Comments

Please visit for bioRxiv preprint.

How to cite this plasmid ( Back to top)

These plasmids were created by your colleagues. Please acknowledge the Principal Investigator, cite the article in which the plasmids were described, and include Addgene in the Materials and Methods of your future publications.

  • For your Materials & Methods section:

    pTRIP TRE BI_SEPT9_i3-14-GFP10_GFP11-14-SEPT7 Gmut2 was a gift from Manos Mavrakis (Addgene plasmid # 180361 ; ; RRID:Addgene_180361)
  • For your References section:

    Human septins organize as octamer-based filaments and mediate actin-membrane anchoring in cells. Martins CS, Taveneau C, Castro-Linares G, Baibakov M, Buzhinsky N, Eroles M, Milanovic V, Omi S, Pedelacq JD, Iv F, Bouillard L, Llewellyn A, Gomes M, Belhabib M, Kuzmic M, Verdier-Pinard P, Lee S, Badache A, Kumar S, Chandre C, Brasselet S, Rico F, Rossier O, Koenderink GH, Wenger J, Cabantous S, Mavrakis M. J Cell Biol. 2023 Mar 6;222(3):e202203016. doi: 10.1083/jcb.202203016. Epub 2022 Dec 23. 10.1083/jcb.202203016 PubMed 36562751