SuExp-mPLD3-IgG2a_Fc
(Plasmid
#180376)
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Purposeexpress secretive mouse PLD3-IgG2a-Fc protein in 293F or Expi 293 cells
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Depositing Lab
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Publication
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Sequence Information
Ordering
| Item | Catalog # | Description | Quantity | Price (USD) | |
|---|---|---|---|---|---|
| Plasmid | 180376 | Standard format: Plasmid sent in bacteria as agar stab | 1 | $94 | |
Backbone
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Vector backboneSuExp
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Backbone manufacturerNemazee lab
- Backbone size w/o insert (bp) 2000
- Total vector size (bp) 4000
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Vector typeMammalian Expression
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Selectable markersZeocin
Growth in Bacteria
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Bacterial Resistance(s)Bleocin (Zeocin), 50 μg/mL
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Growth Temperature37°C
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Growth Strain(s)DH5alpha
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Copy numberHigh Copy
Gene/Insert
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Gene/Insert namePLD3
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SpeciesM. musculus (mouse)
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Insert Size (bp)1300
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Mutationdelete intracellular and transmembrane domain, add signal peptide
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Entrez GenePld3 (a.k.a. Sam-9)
- Promoter CMV
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Tag
/ Fusion Protein
- mouse IgG2a Fc domain (C terminal on insert)
Cloning Information
- Cloning method Ligation Independent Cloning
- 5′ sequencing primer CMV-F
- 3′ sequencing primer SV40-R
- (Common Sequencing Primers)
Terms and Licenses
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Academic/Nonprofit Terms
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Industry Terms
- Not Available to Industry
Trademarks:
- Zeocin® is an InvivoGen trademark.
These plasmids were created by your colleagues. Please acknowledge the Principal Investigator, cite the article in which the plasmids were described, and include Addgene in the Materials and Methods of your future publications.
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For your Materials & Methods section:
SuExp-mPLD3-IgG2a_Fc was a gift from David Nemazee (Addgene plasmid # 180376 ; http://n2t.net/addgene:180376 ; RRID:Addgene_180376)