p17xQUAS-Luciferase, α-cry:mCherry
(Plasmid
#180482)
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PurposeInducible gene expression vector p17xQUAS-Luciferase, α-cry:mCherry
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Depositing Lab
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Depositing OrganizationChungnam National University
Located in Republic of Korea -
Sequence Information
Ordering
| Item | Catalog # | Description | Quantity | Price (USD) | |
|---|---|---|---|---|---|
| Plasmid | 180482 | Standard format: Plasmid sent in bacteria as agar stab | 1 | $94 | |
| DNA | 180482-D50 | 50 μg of DNA in Tris buffer | $495 | ||
| DNA | 180482-D100 | 100 μg of DNA in Tris buffer | $585 | ||
Backbone
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Vector backbonep5xQUAS-ZsGreen-P2A, α-cry:mCherry
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Backbone manufacturerHyunju Ro
- Backbone size w/o insert (bp) 7203
- Total vector size (bp) 8273
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Vector typeSynthetic Biology
Growth in Bacteria
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Bacterial Resistance(s)Ampicillin, 100 μg/mL
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Growth Temperature37°C
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Growth Strain(s)DH5alpha
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Copy numberHigh Copy
Gene/Insert 1
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Gene/Insert nameLuciferase
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Alt namefirefly luciferase
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SpeciesFirefly
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Insert Size (bp)1653
Cloning Information for Gene/Insert 1
- Cloning method Ligation Independent Cloning
- 5′ sequencing primer gtaaaacgacggccagt
- 3′ sequencing primer atctcaacagtctccaca
- (Common Sequencing Primers)
Gene/Insert 2
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Gene/Insert name17x QUAS
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SpeciesSynthetic
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Insert Size (bp)413
Cloning Information for Gene/Insert 2
- Cloning method Ligation Independent Cloning
- 5′ sequencing primer gtaaaacgacggccagt
- 3′ sequencing primer GAGGAGCTCAAAGTGAGG
- (Common Sequencing Primers)
Resource Information
Terms and Licenses
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Academic/Nonprofit Terms
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Industry Terms
- Not Available to Industry
Trademarks:
- Zeocin® is an InvivoGen trademark.
Depositor Comments
Plasmid construct for binary IQ-Switch transgenesis
DNA (Catalog # 180482-D50)
Purpose
Ready-to-use, high-purity DNA prep. DNA aliquots are suitable for use in mammalian cell transfections and other molecular biology applications.
Delivery
- Amount 50 μg
- Concentration 1 μg/μL
- Pricing $495 USD
- Storage 4 ℃ (short-term) or -20 ℃ (long-term)
Terms and Licenses
-
Academic/Nonprofit Terms
-
Industry Terms
- Not Available to Industry
Quality Control
Every DNA prep is verified by next-generation sequencing, confirming plasmid identity, sequence integrity, and absence of DNA contaminants. Concentration is confirmed to be within ±2.5% of the stated concentration.
Our production process has been validated to consistently yield endotoxin levels below 1.0 EU/μg. Individual lots are not tested for endotoxin.
DNA (Catalog # 180482-D100)
Purpose
Ready-to-use, high-purity DNA prep. DNA aliquots are suitable for use in mammalian cell transfections and other molecular biology applications.
Delivery
- Amount 100 μg
- Concentration 1 μg/μL
- Pricing $585 USD
- Storage 4 ℃ (short-term) or -20 ℃ (long-term)
Terms and Licenses
-
Academic/Nonprofit Terms
-
Industry Terms
- Not Available to Industry
Quality Control
Every DNA prep is verified by next-generation sequencing, confirming plasmid identity, sequence integrity, and absence of DNA contaminants. Concentration is confirmed to be within ±2.5% of the stated concentration.
Our production process has been validated to consistently yield endotoxin levels below 1.0 EU/μg. Individual lots are not tested for endotoxin.
These plasmids were created by your colleagues. Please acknowledge the Principal Investigator, cite the article in which the plasmids were described, and include Addgene in the Materials and Methods of your future publications.
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For your Materials & Methods section:
p17xQUAS-Luciferase, α-cry:mCherry was a gift from Hyunju Ro (Addgene plasmid # 180482 ; http://n2t.net/addgene:180482 ; RRID:Addgene_180482) -
For your References section:
IQ-Switch is a QF-based innocuous, silencing-free, and inducible gene switch system in zebrafish. Hong J, Lee JG, Sohn KC, Lee K, Lee S, Lee J, Hong J, Choi D, Hong Y, Jin HS, Choi DK, Lee SU, Kee Y, Jung J, Bae YK, Hwang RH, Hur GM, Lee JS, Ro H. Commun Biol. 2021 Dec 16;4(1):1405. doi: 10.1038/s42003-021-02923-3. 10.1038/s42003-021-02923-3 PubMed 34916605
