pBTK622
(Plasmid #180592)

• Purpose: tdk/kanR dual selection cassette for engineering the genome of Acinetobacter baylyi ADP1
• Depositing Lab: Jeffrey Barrick
• Publication: Suarez et al Nucleic Acids Res. 2020 May 7;48(8):4585-4600. doi: 10.1093/nar/gkaa204.

Backbone

• Vector backbone: ColE1
• Vector type: Synthetic Biology

Growth in Bacteria

• Bacterial Resistance(s): Chloramphenicol, 25 μg/mL
• Growth Temperature: 37°C
• Growth Strain(s): DH5alpha
• Copy number: High Copy

Gene/Insert 1

• Gene/Insert name: tdk
• Species: Escherichia coli
• Insert Size (bp): 618

Gene/Insert 2

• Gene/Insert name: kanR
• Alt name: aphA1
• Species: Various Bacteria
• Insert Size (bp): 816

Resource Information

• Supplemental Documents:
  • pbtk622-tdkkanr.gb
• A portion of this plasmid was derived from a plasmid made by: tdk-KanR cassette from Valerie de Crecy-Lagard at the University of Florida. This cassette is in general usage in many labs studying Acinetobacter baylyi ADP1.

Terms and Licenses

• Academic/Nonprofit Terms:
  • UBMTA
• Industry Terms:
  • Not Available to Industry

Depositor Comments

The tdk-KanR was decribed in Metzgar, David, Jamie M Bacher, Valérie Pezo, John Reader, Volker Döring, Paul Schimmel, Philippe Marlière, and Valérie de Crécy-Lagard. (2004) Acinetobacter Sp. ADP1: An Ideal Model Organism for Genetic Analysis and Genome Engineering. Nucleic Acids Research 32 (19): 5780–5790. https://doi.org/10.1093/nar/gkh881.