pEGFP/GCP60
(Plasmid
#180633)
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PurposeExpression of GCP60
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Depositing Lab
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Sequence Information
Ordering
| Item | Catalog # | Description | Quantity | Price (USD) | |
|---|---|---|---|---|---|
| Plasmid | 180633 | Standard format: Plasmid sent in bacteria as agar stab | 1 | $85 | |
This material is available to academics and nonprofits only.
Backbone
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Vector backbonepEGFP-C3
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Backbone manufacturerClontech
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Vector typeMammalian Expression
Growth in Bacteria
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Bacterial Resistance(s)Kanamycin, 50 μg/mL
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Growth Temperature37°C
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Growth Strain(s)DH5alpha
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Copy numberHigh Copy
Gene/Insert
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Gene/Insert nameGCP60
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Alt nameACBD3
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SpeciesH. sapiens (human)
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Entrez GeneACBD3 (a.k.a. GCP60, GOCAP1, GOLPH1, PAP7)
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Tag
/ Fusion Protein
- EGFP (N terminal on backbone)
Cloning Information
- Cloning method Restriction Enzyme
- 5′ cloning site Unknown (unknown if destroyed)
- 3′ cloning site Unknown (unknown if destroyed)
Terms and Licenses
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Academic/Nonprofit Terms
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Industry Terms
- Not Available to Industry
Trademarks:
- Zeocin® is an InvivoGen trademark.
How to cite this plasmid
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These plasmids were created by your colleagues. Please acknowledge the Principal Investigator, cite the article in which the plasmids were described, and include Addgene in the Materials and Methods of your future publications.
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For your Materials & Methods section:
pEGFP/GCP60 was a gift from Carolyn Machamer (Addgene plasmid # 180633 ; http://n2t.net/addgene:180633 ; RRID:Addgene_180633) -
For your References section:
GCP60 preferentially interacts with a caspase-generated golgin-160 fragment. Sbodio JI, Hicks SW, Simon D, Machamer CE. J Biol Chem. 2006 Sep 22;281(38):27924-31. doi: 10.1074/jbc.M603276200. Epub 2006 Jul 26. 10.1074/jbc.M603276200 PubMed 16870622
