Full plasmid sequence is not available for this item.
|Item||Catalog #||Description||Quantity||Price (USD)|
|Plasmid||18068||Standard format: Plasmid sent in bacteria as agar stab||1||$75|
This material is available to academics and nonprofits only.
Backbone manufacturergift from Japan
- Backbone size w/o insert (bp) 6200
Vector typeMammalian Expression
Selectable markersNeomycin (select with G418)
Growth in Bacteria
Bacterial Resistance(s)Ampicillin, 100 μg/mL
Copy numberHigh Copy
Gene/Insert nameyes kinase
SpeciesM. musculus (mouse)
Insert Size (bp)1800
MutationTyrosine 535 changed to Phenylalanine
Entrez GeneYes1 (a.k.a. Yes, p61-Yes)
- Cloning method Restriction Enzyme
- 5′ cloning site MluI (destroyed during cloning)
- 3′ cloning site MluI (destroyed during cloning)
- 5′ sequencing primer N/A (Common Sequencing Primers)
Terms and Licenses
- Not Available to Industry
- Zeocin® is an InvivoGen trademark.
The vector pMIK-Neo is a gift from Dr. Kazuo Maruyama of the University of Tokyo, Tokyo, Japan. The map of the vector will be sent with the DNA
These plasmids were created by your colleagues. Please acknowledge the Principal Investigator, cite the article in which the plasmids were described, and include Addgene in the Materials and Methods of your future publications.
For your Materials & Methods section:pMIK-Neo-mYes-Y535F was a gift from Marius Sudol (Addgene plasmid # 18068 ; http://n2t.net/addgene:18068 ; RRID:Addgene_18068)
For your References section:Yes-associated protein and p53-binding protein-2 interact through their WW and SH3 domains. Espanel X, Sudol M. J Biol Chem. 2001 Apr 27. 276(17):14514-23. 10.1074/jbc.M008568200 PubMed 11278422
Map uploaded by the depositor.