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pMIK-Neo-mYes-Y535F
(Plasmid #18068)

Full plasmid sequence is not available for this item.

Ordering

Item Catalog # Description Quantity Price (USD)
Plasmid 18068 Standard format: Plasmid sent in bacteria as agar stab 1 $65

This material is available to academics and nonprofits only.

Backbone

  • Vector backbone
    pMIK-Neo
  • Backbone manufacturer
    gift from Japan
  • Backbone size w/o insert (bp) 6200
  • Vector type
    Mammalian Expression
  • Selectable markers
    Neomycin (select with G418)

Growth in Bacteria

  • Bacterial Resistance(s)
    Ampicillin
  • Growth Temperature
    37°C
  • Growth Strain(s)
    DH5alpha
  • Copy number
    High Copy

Gene/Insert

  • Gene/Insert name
    yes kinase
  • Alt name
    c-yes
  • Species
    M. musculus (mouse)
  • Insert Size (bp)
    1800
  • Mutation
    Tyrosine 535 changed to Phenylalanine
  • GenBank ID
    NM_009535
  • Entrez Gene
    Yes1 (a.k.a. Yes, p61-Yes)

Cloning Information

  • Cloning method Restriction Enzyme
  • 5′ cloning site MluI (destroyed during cloning)
  • 3′ cloning site MluI (destroyed during cloning)
  • 5′ sequencing primer N/A
  • (Common Sequencing Primers)

Resource Information

  • A portion of this plasmid was derived from a plasmid made by
    The mouse "yes" cDNA gene was a gift from Dr. J. Bolen (Oncogene 1993, Volume 8, pages 713-719, the first author: Klages, S.
  • Terms and Licenses

Depositor Comments

The vector pMIK-Neo is a gift from Dr. Kazuo Maruyama of the University of Tokyo, Tokyo, Japan. The map of the vector will be sent with the DNA

How to cite this plasmid ( Back to top)

These plasmids were created by your colleagues. Please acknowledge the Principal Investigator, cite the article in which the plasmids were described, and include Addgene in the Materials and Methods of your future publications.

  • For your Materials & Methods section:

    pMIK-Neo-mYes-Y535F was a gift from Marius Sudol (Addgene plasmid # 18068 ; http://n2t.net/addgene:18068 ; RRID:Addgene_18068)
  • For your References section:

    Yes-associated protein and p53-binding protein-2 interact through their WW and SH3 domains. Espanel X, Sudol M. J Biol Chem. 2001 Apr 27. 276(17):14514-23. 10.1074/jbc.M008568200 PubMed 11278422