pSUMO-β′-COPI-WD40
(Plasmid #180753)

• Purpose: Bacterial expression plasmid for Saccharomyces cerevisiae β′-COPI-WD40 (residues 1-301).
• Depositing Lab: Syed Saif Hasan
• Publication: Dey et al Commun Biol. 2022 Feb 8;5(1):115. doi: 10.1038/s42003-022-03063-y.

Backbone

• Vector backbone: pSUMO
• Backbone size w/o insert (bp): 5731
• Vector type: Bacterial Expression

Growth in Bacteria

• Bacterial Resistance(s): Ampicillin, 100 μg/mL
• Growth Temperature: 37°C
• Growth Strain(s): DH5alpha
• Copy number: Unknown

Gene/Insert

• Gene/Insert name: β′-COPI-WD40
• Species: S. cerevisiae (budding yeast)
• Mutation: Encodes residues 1-301; deleted 302-889; mutated Leu39 to Ile39
• GenBank ID: NM_001181002.1
• Entrez Gene: SEC27 (a.k.a. YGL137W)
• Promoter: T7
• Tag / Fusion Protein:
  • N-terminal strep tag, His-tag, and SUMO tag (N terminal on insert)

Cloning Information

• Cloning method: Ligation Independent Cloning
• 5′ sequencing primer: T7 promoter
• 3′ sequencing primer: T7 terminator

Resource Information

• Article Citing this Plasmid:
  • 1 Reference

Terms and Licenses

• Academic/Nonprofit Terms:
  • UBMTA
• Industry Terms:
  • Not Available to Industry