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pSUMO-β′-COPI-WD40
(Plasmid #180753)

Ordering

Item Catalog # Description Quantity Price (USD)
Plasmid 180753 Standard format: Plasmid sent in bacteria as agar stab 1 $75

This material is available to academics and nonprofits only.

Backbone

Growth in Bacteria

  • Bacterial Resistance(s)
    Ampicillin, 100 μg/mL
  • Growth Temperature
    37°C
  • Growth Strain(s)
    DH5alpha
  • Copy number
    Unknown

Gene/Insert

  • Gene/Insert name
    β′-COPI-WD40
  • Species
    S. cerevisiae (budding yeast)
  • Mutation
    Encodes residues 1-301; deleted 302-889; mutated Leu39 to Ile39
  • GenBank ID
    NM_001181002.1
  • Entrez Gene
    SEC27 (a.k.a. YGL137W)
  • Promoter T7
  • Tag / Fusion Protein
    • N-terminal strep tag, His-tag, and SUMO tag (N terminal on insert)

Cloning Information

  • Cloning method Ligation Independent Cloning
  • 5′ sequencing primer T7 promoter
  • 3′ sequencing primer T7 terminator
  • (Common Sequencing Primers)

Terms and Licenses

  • Academic/Nonprofit Terms
  • Industry Terms
    • Not Available to Industry
Trademarks:
  • Zeocin® is an InvivoGen trademark.
How to cite this plasmid ( Back to top)

These plasmids were created by your colleagues. Please acknowledge the Principal Investigator, cite the article in which the plasmids were described, and include Addgene in the Materials and Methods of your future publications.

  • For your Materials & Methods section:

    pSUMO-β′-COPI-WD40 was a gift from Syed Saif Hasan (Addgene plasmid # 180753 ; http://n2t.net/addgene:180753 ; RRID:Addgene_180753)
  • For your References section:

    An extended motif in the SARS-CoV-2 spike modulates binding and release of host coatomer in retrograde trafficking. Dey D, Singh S, Khan S, Martin M, Schnicker NJ, Gakhar L, Pierce BG, Hasan SS. Commun Biol. 2022 Feb 8;5(1):115. doi: 10.1038/s42003-022-03063-y. 10.1038/s42003-022-03063-y PubMed 35136165