Skip to main content
This website uses cookies to ensure you get the best experience. By continuing to use this site, you agree to the use of cookies.
We have implemented updates to our Transparency and Privacy Policy.

Please note: Your browser does not support the features used on Addgene's website. You may not be able to create an account or request plasmids through this website until you upgrade your browser. Learn more

Please note: Your browser does not fully support some of the features used on Addgene's website. If you run into any problems registering, depositing, or ordering please contact us at [email protected] Learn more

(Plasmid #180753)


Item Catalog # Description Quantity Price (USD)
Plasmid 180753 Standard format: Plasmid sent in bacteria as agar stab 1 $75

This material is available to academics and nonprofits only.


Growth in Bacteria

  • Bacterial Resistance(s)
    Ampicillin, 100 μg/mL
  • Growth Temperature
  • Growth Strain(s)
  • Copy number


  • Gene/Insert name
  • Species
    S. cerevisiae (budding yeast)
  • Mutation
    Encodes residues 1-301; deleted 302-889; mutated Leu39 to Ile39
  • GenBank ID
  • Entrez Gene
    SEC27 (a.k.a. YGL137W)
  • Promoter T7
  • Tag / Fusion Protein
    • N-terminal strep tag, His-tag, and SUMO tag (N terminal on insert)

Cloning Information

  • Cloning method Ligation Independent Cloning
  • 5′ sequencing primer T7 promoter
  • 3′ sequencing primer T7 terminator
  • (Common Sequencing Primers)

Terms and Licenses

  • Academic/Nonprofit Terms
  • Industry Terms
    • Not Available to Industry
  • Zeocin® is an InvivoGen trademark.
How to cite this plasmid ( Back to top)

These plasmids were created by your colleagues. Please acknowledge the Principal Investigator, cite the article in which the plasmids were described, and include Addgene in the Materials and Methods of your future publications.

  • For your Materials & Methods section:

    pSUMO-β′-COPI-WD40 was a gift from Syed Saif Hasan (Addgene plasmid # 180753 ; ; RRID:Addgene_180753)
  • For your References section:

    An extended motif in the SARS-CoV-2 spike modulates binding and release of host coatomer in retrograde trafficking. Dey D, Singh S, Khan S, Martin M, Schnicker NJ, Gakhar L, Pierce BG, Hasan SS. Commun Biol. 2022 Feb 8;5(1):115. doi: 10.1038/s42003-022-03063-y. 10.1038/s42003-022-03063-y PubMed 35136165