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pRQ-hRasV12
(Plasmid #18113)

Ordering

Item Catalog # Description Quantity Price (USD)
Plasmid 18113 Standard format: Plasmid sent in bacteria as agar stab 1 $75

This material is available to academics and nonprofits only.

Backbone

  • Vector backbone
    N/A
  • Backbone manufacturer
    Constructed from multiple sources
  • Backbone size w/o insert (bp) 6664
  • Vector type
    Mammalian Expression, Retroviral
  • Selectable markers
    Puromycin

Growth in Bacteria

  • Bacterial Resistance(s)
    Ampicillin
  • Growth Temperature
    30°C
  • Growth Strain(s)
    Stbl3
  • Growth instructions
    STBL2 30'C
  • Copy number
    Low Copy

Gene/Insert

  • Gene/Insert name
    h-RasV12G
  • Alt name
    harvey Ras
  • Alt name
    oncogenic ras
  • Species
    H. sapiens (human)
  • Insert Size (bp)
    569
  • Mutation
    G12V, constitutively active
  • GenBank ID
    NM_005343
  • Entrez Gene
    HRAS (a.k.a. C-BAS/HAS, C-H-RAS, C-HA-RAS1, CTLO, H-RASIDX, HAMSV, HRAS1, RASH1, p21ras)

Cloning Information

  • Cloning method Gateway Cloning
  • 5′ cloning site Gateway (unknown if destroyed)
  • 3′ cloning site gateway (unknown if destroyed)
  • 5′ sequencing primer GCT CGT TTA GTG AAC CGT CA
  • (Common Sequencing Primers)

Resource Information

  • Terms and Licenses
  • Industry Terms
    • Not Available to Industry
How to cite this plasmid ( Back to top)

These plasmids were created by your colleagues. Please acknowledge the Principal Investigator, cite the article in which the plasmids were described, and include Addgene in the Materials and Methods of your future publications.

  • For your Materials & Methods section:

    pRQ-hRasV12 was a gift from Alex Swarbrick (Addgene plasmid # 18113 ; http://n2t.net/addgene:18113 ; RRID:Addgene_18113)
  • For your References section:

    Id1 cooperates with oncogenic Ras to induce metastatic mammary carcinoma by subversion of the cellular senescence response. Swarbrick A, Roy E, Allen T, Bishop JM. Proc Natl Acad Sci U S A. 2008 Apr 8. 105(14):5402-7. 10.1073/pnas.0801505105 PubMed 18378907