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Addgene

pCMV-MMLVgag-3xNES-ABE8e Notes

Addgene Diagnostic Digest

This gel depicts the NEB 1kb Extended Ladder and a digestion of the plasmid pCMV-MMLVgag-3xNES-ABE8e with the following enzymes: DraIII-HF, EcoRV-HF

Expected bands:
DraIII-HF: 6.7 + 4.6 kb (checking for signs of cross-contamination with a supercoiled ~4.8kb plasmid)
EcoRV-HF: 11.4 (checking for signs of cross-contamination with a single cut ~4.8kb plasmid)

Results:
We observed the expected bands for these digests.