MUM2SP-Citrine
(Plasmid #181963)

• Purpose: MUM2 signal peptide fused to Citrine, under control of MUM4_1.5kb promoter
• Depositing Lab: George Haughn
• Publication: Lee et al Plant Direct. 2021 Nov 28;5(11):e360. doi: 10.1002/pld3.360. eCollection 2021 Nov.

Backbone

• Vector backbone: pAD
• Backbone manufacturer: DeBono 2011
• Backbone size w/o insert (bp): 5000
• Total vector size (bp): 6500
• Vector type: Plant Expression
• Selectable markers: Basta

Growth in Bacteria

• Bacterial Resistance(s): Kanamycin, 50 μg/mL
• Growth Temperature: 37°C
• Growth Strain(s): DH5alpha
• Growth instructions: pAD is based on pGreenII 0229. The pSOUP helper is required for transformation of plant tissue
• Copy number: High Copy

Gene/Insert

• Gene/Insert name: MUM2 signal peptide, fused to the Citrine YFP in pAD
• Species: A. thaliana (mustard weed)
• Insert Size (bp): 83
• GenBank ID: At5g63800
• Promoter: MUM4pro 1.5kb fragment
• Tag / Fusion Protein:
  • Citrine YFP (C terminal on backbone)

Cloning Information

• Cloning method: Restriction Enzyme
• 5′ cloning site: KnpI (not destroyed)
• 3′ cloning site: XhoI (not destroyed)
• 5′ sequencing primer: MUM4 promoter Rev
• 3′ sequencing primer: Citrine REv GGACACGCTGAACTTGTGG

Terms and Licenses

• Academic/Nonprofit Terms:
  • UBMTA
• Industry Terms:
  • Not Available to Industry