pEGFP-GFP11-Actin
(Plasmid #181966)

• Purpose: Human beta actin tagged with GFP11.
• Depositing Lab: Jin Zhang
• Publication: Tenner et al Sci Adv. 2021 May 21;7(21). pii: 7/21/eabe4091. doi: 10.1126/sciadv.abe4091. Print 2021 May.

Backbone

• Vector backbone: pEGFP
• Backbone size w/o insert (bp): 3954
• Total vector size (bp): 5184
• Vector type: Mammalian Expression
• Selectable markers: Neomycin (select with G418)

Growth in Bacteria

• Bacterial Resistance(s): Kanamycin, 50 μg/mL
• Growth Temperature: 37°C
• Growth Strain(s): DH5alpha
• Copy number: High Copy

Gene/Insert

• Gene/Insert name: GFP11-Actin
• Species: H. sapiens (human)
• Insert Size (bp): 1230
• Entrez Gene: ACTB (a.k.a. BKRNS, BNS, BRWS1, CSMH, DDS1, PS1TP5BP1, THC8)
• Promoter: CMV
• Tag / Fusion Protein:
  • GFP11 (N terminal on insert)

Cloning Information

• Cloning method: Restriction Enzyme
• 5′ cloning site: AgeI (not destroyed)
• 3′ cloning site: BamHI (not destroyed)
• 5′ sequencing primer: CMV forward
• 3′ sequencing primer: SV40pA reverse

Resource Information

• Supplemental Documents:
  • pEGFP-GFP11-Actin.gb
• A portion of this plasmid was derived from a plasmid made by: Plasmid generated by Bo Huang (UCSF).

Terms and Licenses

• Academic/Nonprofit Terms:
  • UBMTA
• Industry Terms:
  • Not Available to Industry