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pmiRGLO Dual-Luciferase-PDAP1-3'UTR-Mut4x
(Plasmid #182256)

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This material is available to academics and nonprofits only.
Item Catalog # Description Quantity Price (USD)
Plasmid 182256 Standard format: Plasmid sent in bacteria as agar stab 1 $94
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DNA 182256-D50 50 μg of DNA in Tris buffer $495
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DNA 182256-D100 100 μg of DNA in Tris buffer $585
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Backbone

  • Vector backbone
    pmirGLO Dual-Luciferase
  • Backbone manufacturer
    Promega
  • Backbone size w/o insert (bp) 7344
  • Total vector size (bp) 7946
  • Vector type
    Mammalian Expression, Luciferase

Growth in Bacteria

  • Bacterial Resistance(s)
    Ampicillin, 100 μg/mL
  • Growth Temperature
    37°C
  • Growth Strain(s)
    DH5alpha
  • Copy number
    High Copy

Gene/Insert

  • Gene/Insert name
    3' UTR region of PDAP1 gene mutated in 4 binding sites for mir-150
  • Species
    H. sapiens (human)
  • Insert Size (bp)
    604

Cloning Information

  • Cloning method Restriction Enzyme
  • 5′ cloning site NheI (unknown if destroyed)
  • 3′ cloning site XbaI (unknown if destroyed)
  • 5′ sequencing primer AGA AGC TGC GCG GTG GTG TTG TG
  • (Common Sequencing Primers)

Terms and Licenses

Trademarks:

  • Zeocin® is an InvivoGen trademark.

Depositor Comments

Please note there is a L95R mutation in hRluc. Depositor confirms this does not affect plasmid function.

Purpose

Ready-to-use, high-purity DNA prep. DNA aliquots are suitable for use in mammalian cell transfections and other molecular biology applications.

Delivery

  • Amount 50 μg
  • Concentration 1 μg/μL
  • Pricing $495 USD
  • Storage 4 ℃ (short-term) or -20 ℃ (long-term)

Terms and Licenses

Quality Control

Every DNA prep is verified by next-generation sequencing, confirming plasmid identity, sequence integrity, and absence of DNA contaminants. Concentration is confirmed to be within ±2.5% of the stated concentration.

Our production process has been validated to consistently yield endotoxin levels below 1.0 EU/μg. Individual lots are not tested for endotoxin.

Purpose

Ready-to-use, high-purity DNA prep. DNA aliquots are suitable for use in mammalian cell transfections and other molecular biology applications.

Delivery

  • Amount 100 μg
  • Concentration 1 μg/μL
  • Pricing $585 USD
  • Storage 4 ℃ (short-term) or -20 ℃ (long-term)

Terms and Licenses

Quality Control

Every DNA prep is verified by next-generation sequencing, confirming plasmid identity, sequence integrity, and absence of DNA contaminants. Concentration is confirmed to be within ±2.5% of the stated concentration.

Our production process has been validated to consistently yield endotoxin levels below 1.0 EU/μg. Individual lots are not tested for endotoxin.

How to cite this plasmid ( Back to top)

These plasmids were created by your colleagues. Please acknowledge the Principal Investigator, cite the article in which the plasmids were described, and include Addgene in the Materials and Methods of your future publications.

  • For your Materials & Methods section:

    pmiRGLO Dual-Luciferase-PDAP1-3'UTR-Mut4x was a gift from Silvia Monticelli (Addgene plasmid # 182256 ; http://n2t.net/addgene:182256 ; RRID:Addgene_182256)

  • For your References section:

    RFX transcription factors control a miR-150/PDAP1 axis that restrains the proliferation of human T cells. Chirichella M, Bianchi N, Dzafo E, Foli E, Gualdrini F, Kenyon A, Natoli G, Monticelli S. PLoS Biol. 2022 Feb 10;20(2):e3001538. doi: 10.1371/journal.pbio.3001538. 10.1371/journal.pbio.3001538 PubMed 35143476
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