pSEM397 - mosTI unc-119 - NNN - operon - gfp - nls - tbb-2
(Plasmid
#182354)
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Purpose(Empty Backbone) Nuclear GFP co-expression cloning vector for mosTI(unc-119). Transgene inserted upstream of gpd-2 operon::gfp(2xNLS)::tbb-2 3'UTR. MCS or Golden Gate (BsaI: tgcc - MCS - tgag)
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Depositing Lab
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Sequence Information
Ordering
| Item | Catalog # | Description | Quantity | Price (USD) | |
|---|---|---|---|---|---|
| Plasmid | 182354 | Standard format: Plasmid sent in bacteria as agar stab | 1 | $85 | |
This material is available to academics and nonprofits only.
Backbone
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Vector backbonepTwist-Amp-high-copy
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Backbone manufacturerTwist Biosciences
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Vector typeWorm Expression
Growth in Bacteria
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Bacterial Resistance(s)Ampicillin, 100 μg/mL
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Growth Temperature37°C
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Growth Strain(s)DH5alpha
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Copy numberHigh Copy
Resource Information
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Supplemental Documents
Terms and Licenses
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Academic/Nonprofit Terms
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Industry Terms
- Not Available to Industry
Trademarks:
- Zeocin® is an InvivoGen trademark.
Depositor Comments
www.wormbuilder.org
MOdular Safe harbor Transgene Insertion (mosTI).
Cloning vector for single-copy insertion using unc-119 selection.
MCS/Golden Gate (BsaI) cloning vector with insertion between upstream of gpd-2 operon and gfp(NLS)::tbb-2 3' UTR.
Use to monitor expression of transgene by co-expression of fluorophore.
BsaI overhangs: tgcc - mcs - tgag (use tga as the stop site in the BsaI overlap for transgenes).
Please visit https://www.biorxiv.org/content/10.1101/2022.04.19.488726v1.full for bioRxiv preprint.
These plasmids were created by your colleagues. Please acknowledge the Principal Investigator, cite the article in which the plasmids were described, and include Addgene in the Materials and Methods of your future publications.
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For your Materials & Methods section:
pSEM397 - mosTI unc-119 - NNN - operon - gfp - nls - tbb-2 was a gift from Christian Froekjaer-Jensen (Addgene plasmid # 182354 ; http://n2t.net/addgene:182354 ; RRID:Addgene_182354) -
For your References section:
Modular safe-harbor transgene insertion (MosTI) for targeted single-copy and extrachromosomal array integration in C. elegans. El Mouridi S, Alkhaldi F, Frokjaer-Jensen C. G3 (Bethesda). 2022 Jul 28. pii: 6651068. doi: 10.1093/g3journal/jkac184. 10.1093/g3journal/jkac184 PubMed 35900171
Map uploaded by the depositor.
Map uploaded by the depositor.
