deltaVP1d63C + VP2C-GFPdeg
(Plasmid #182481)

• Purpose: YIp for expressing Murine polyomavirus deltaVP1 with the 63 C-ter residues truncated (GAL1 promoter) and destabilised GFP tagged with VP2C (GAL10 promoter). Contains uracil marker (K. lactis URA3).
• Depositing Lab: Claudia Vickers
• Publication: Li Chen Cheah et al BioRxiv, 2022

Backbone

• Vector backbone: pILGFPB5A
• Vector type: Yeast Expression, Synthetic Biology ; Metabolic engineering
• Selectable markers: URA3

Growth in Bacteria

• Bacterial Resistance(s): Ampicillin, 100 μg/mL
• Growth Temperature: 37°C
• Growth Strain(s): DH5alpha
• Copy number: High Copy

Gene/Insert 1

• Gene/Insert name: VP2C-GFPdeg
• Alt name: VP2C-yEGFPdeg, VP2C-GFPmodc, destabilised GFP, degron-tagged GFP
• Species: M. musculus (mouse), Synthetic; Murine polyomavirus
• Insert Size (bp): 1038
• Promoter: GAL10

Cloning Information for Gene/Insert 1

• Cloning method: Gibson Cloning
• 5′ sequencing primer: GTGGTAATGCCATGTAATATGATTATTAAAC
• 3′ sequencing primer: GCATTGGCACGGTGCAACAC

Gene/Insert 2

• Gene/Insert name: deltaVP1d63C
• Alt name: VP1, deltaNLS VP1, C-terminal truncated VP1
• Species: Synthetic; Murine polyomavirus
• Insert Size (bp): 954
• Promoter: GAL1

Cloning Information for Gene/Insert 2

• Cloning method: Gibson Cloning
• 5′ sequencing primer: ACTTCTTATTCAAATGTCATAAAAGTATCAAC
• 3′ sequencing primer: CCAGCCTGCTTTTCTGTAAC

Resource Information

• Supplemental Documents:
  • deltaVP1d63C + VP2C-GFPdeg.gb

Terms and Licenses

• Academic/Nonprofit Terms:
  • UBMTA
• Industry Terms:
  • Not Available to Industry

Depositor Comments

VP1 has 63 residues removed from the C-terminal. This abolishes in-vivo assembly in yeast.

Please visit https://www.biorxiv.org/content/10.1101/2022.11.24.517869v1 for bioRxiv preprint.