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pLenti GW V5 Eco/Dam hum LaminA
(Plasmid #182673)

Ordering

Item Catalog # Description Quantity Price (USD)
Plasmid 182673 Standard format: Plasmid sent in bacteria as agar stab 1 $85

This material is available to academics and nonprofits only.

Backbone

  • Vector backbone
    pLenti Gateway
  • Backbone size w/o insert (bp) 8589
  • Total vector size (bp) 10370
  • Vector type
    Mammalian Expression, Lentiviral
  • Selectable markers
    bleomycin

Growth in Bacteria

  • Bacterial Resistance(s)
    Ampicillin, 100 μg/mL
  • Growth Temperature
    37°C
  • Growth Strain(s)
    NEB Stable
  • Copy number
    Unknown

Gene/Insert

  • Gene/Insert name
    Lamin A
  • Species
    H. sapiens (human)
  • Insert Size (bp)
    1750
  • Entrez Gene
    LMNA (a.k.a. CDCD1, CDDC, CMD1A, CMT2B1, EMD2, FPL, FPLD, FPLD2, HGPS, IDC, LDP1, LFP, LGMD1B, LMN1, LMNC, LMNL1, MADA, PRO1)
  • Promoter HSP
  • Tags / Fusion Proteins
    • E. Coli Dam (N terminal on insert)
    • V5 (N terminal on insert)

Cloning Information

Terms and Licenses

  • Academic/Nonprofit Terms
  • Industry Terms
    • Not Available to Industry
Trademarks:
  • Zeocin® is an InvivoGen trademark.

Depositor Comments

Addgene QC NGS analysis returned BLAST results with significant sequence identity to Lamin A/C.

How to cite this plasmid ( Back to top)

These plasmids were created by your colleagues. Please acknowledge the Principal Investigator, cite the article in which the plasmids were described, and include Addgene in the Materials and Methods of your future publications.

  • For your Materials & Methods section:

    pLenti GW V5 Eco/Dam hum LaminA was a gift from Bas van Steensel (Addgene plasmid # 182673 ; http://n2t.net/addgene:182673 ; RRID:Addgene_182673)
  • For your References section:

    Constitutive nuclear lamina-genome interactions are highly conserved and associated with A/T-rich sequence. Meuleman W, Peric-Hupkes D, Kind J, Beaudry JB, Pagie L, Kellis M, Reinders M, Wessels L, van Steensel B. Genome Res. 2013 Feb;23(2):270-80. doi: 10.1101/gr.141028.112. Epub 2012 Nov 2. 10.1101/gr.141028.112 PubMed 23124521