pGWT35S-KON206-MXMT
(Plasmid
#182729)
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PurposeTransient expression of KON206-MXMT in plant cell
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Depositing Lab
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Sequence Information
Ordering
| Item | Catalog # | Description | Quantity | Price (USD) | |
|---|---|---|---|---|---|
| Plasmid | 182729 | Standard format: Plasmid sent in bacteria as agar stab | 1 | $85 | |
This material is available to academics and nonprofits only.
Backbone
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Vector backbonepGWT35S
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Modifications to backbonethe MXMT gene was subcloned into the NcoI/SpeI site of the p35S-sfGFP vectors encoding the BiFC fragments.
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Vector typePlant Expression
Growth in Bacteria
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Bacterial Resistance(s)Kanamycin, 50 μg/mL
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Growth Temperature37°C
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Growth Strain(s)DH5alpha
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Copy numberUnknown
Gene/Insert
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Gene/Insert nameKON206
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Alt nameN-terminal fragment of mKO2, aa1-206
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SpeciesSynthetic
- Promoter CaMV 35S promoter
Cloning Information
- Cloning method Gateway Cloning
- 5′ sequencing primer GGGGACAAGTTTGTACAAAAAAGCAGGCTTCATGGTGAGTGTGATTAAA
- 3′ sequencing primer GGGGACCACTTTGTACAAGAAAGCTGGGTCTTAGTTGCCTTCGGTTTTC (Common Sequencing Primers)
Terms and Licenses
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Academic/Nonprofit Terms
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Industry Terms
- Not Available to Industry
Trademarks:
- Zeocin® is an InvivoGen trademark.
How to cite this plasmid
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These plasmids were created by your colleagues. Please acknowledge the Principal Investigator, cite the article in which the plasmids were described, and include Addgene in the Materials and Methods of your future publications.
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For your Materials & Methods section:
pGWT35S-KON206-MXMT was a gift from Yutaka Kodama (Addgene plasmid # 182729 ; http://n2t.net/addgene:182729 ; RRID:Addgene_182729) -
For your References section:
A novel orange-colored bimolecular fluorescence complementation (BiFC) assay using monomeric Kusabira-Orange protein. Fujii Y, Yoshimura A, Kodama Y. Biotechniques. 2018 Apr;64(4):153-161. doi: 10.2144/btn-2017-0121. 10.2144/btn-2017-0121 PubMed 29661017
