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(Plasmid #182931)


Item Catalog # Description Quantity Price (USD)
Plasmid 182931 Standard format: Plasmid sent in bacteria as agar stab 1 $85

This material is available to academics and nonprofits only.


  • Vector backbone
  • Backbone manufacturer
  • Backbone size (bp) 6140
  • Vector type
    Mammalian Expression
  • Promoter CMV
  • Selectable markers
    Neomycin (select with G418)
  • Tags / Fusion Proteins
    • HA signal peptide (N terminal on insert)
    • FLAG tag (N terminal on insert)
    • 3C-cleavable split GFP 1-10 (C terminal on insert)

Growth in Bacteria

  • Bacterial Resistance(s)
    Ampicillin, 100 μg/mL
  • Growth Temperature
  • Growth Strain(s)
  • Copy number
    High Copy

Cloning Information

Terms and Licenses

  • Academic/Nonprofit Terms
  • Industry Terms
    • Not Available to Industry
  • Zeocin® is an InvivoGen trademark.
How to cite this plasmid ( Back to top)

These plasmids were created by your colleagues. Please acknowledge the Principal Investigator, cite the article in which the plasmids were described, and include Addgene in the Materials and Methods of your future publications.

  • For your Materials & Methods section:

    FLAG-3C-sGFP1-10_pcDNA3.1(-) was a gift from William Weis (Addgene plasmid # 182931 ; ; RRID:Addgene_182931)
  • For your References section:

    Reconstitution of purified membrane protein dimers in lipid nanodiscs with defined stoichiometry and orientation using a split GFP tether. Bruguera ES, Mahoney JP, Weis WI. J Biol Chem. 2022 Jan 21:101628. doi: 10.1016/j.jbc.2022.101628. 10.1016/j.jbc.2022.101628 PubMed 35074428