Skip to main content
This website uses cookies to ensure you get the best experience. By continuing to use this site, you agree to the use of cookies.

Please note: Your browser does not support the features used on Addgene's website. You may not be able to create an account or request plasmids through this website until you upgrade your browser. Learn more

Please note: Your browser does not fully support some of the features used on Addgene's website. If you run into any problems registering, depositing, or ordering please contact us at [email protected]. Learn more


(Plasmid #183078)


Item Catalog # Description Quantity Price (USD)
Plasmid 183078 Standard format: Plasmid sent in bacteria as agar stab 1 $85

This material is available to academics and nonprofits only.


  • Vector backbone
  • Backbone manufacturer
    Received from R. Platt from F. Zhang lab, Broad Institute
  • Backbone size w/o insert (bp) 4153
  • Total vector size (bp) 5990
  • Modifications to backbone
    Replaced all sequence between AAV ITRs with insert acceptance site and PGK-Puro-PolyA.
  • Vector type
    AAV ; Homologous Recombination Donor Template
  • Selectable markers

Growth in Bacteria

  • Bacterial Resistance(s)
    Ampicillin, 100 μg/mL
  • Growth Temperature
  • Growth Strain(s)
    NEB Stable
  • Copy number


  • Gene/Insert name
    BRAF Exon 15, 1.8kb homologous recombination donor, ~900 bp homology arms
  • Alt name
    BRAF Exon 15
  • Species
    H. sapiens (human)
  • Insert Size (bp)
  • Mutation
    V600E (T>A), S607S (TCC>AGT)
  • Entrez Gene
    BRAF (a.k.a. B-RAF1, B-raf, BRAF-1, BRAF1, NS7, RAFB1)
  • Promoter None for primary insert. PGK for puromycin resistance gene.

Cloning Information

  • Cloning method Restriction Enzyme
  • 5′ cloning site MluI (not destroyed)
  • 3′ cloning site AgeI (not destroyed)
  • 5′ sequencing primer NGS
  • 3′ sequencing primer NGS
  • (Common Sequencing Primers)

Resource Information

Terms and Licenses

  • Academic/Nonprofit Terms
  • Industry Terms
    • Not Available to Industry
  • Zeocin® is an InvivoGen trademark.
How to cite this plasmid ( Back to top)

These plasmids were created by your colleagues. Please acknowledge the Principal Investigator, cite the article in which the plasmids were described, and include Addgene in the Materials and Methods of your future publications.

  • For your Materials & Methods section:

    pEH_AAV_BRAF_ex15_V600E_S607S_1.8kb was a gift from Aviv Regev (Addgene plasmid # 183078 ; ; RRID:Addgene_183078)
  • For your References section:

    Stepwise-edited, human melanoma models reveal mutations' effect on tumor and microenvironment. Hodis E, Torlai Triglia E, Kwon JYH, Biancalani T, Zakka LR, Parkar S, Hutter JC, Buffoni L, Delorey TM, Phillips D, Dionne D, Nguyen LT, Schapiro D, Maliga Z, Jacobson CA, Hendel A, Rozenblatt-Rosen O, Mihm MC Jr, Garraway LA, Regev A. Science. 2022 Apr 29;376(6592):eabi8175. doi: 10.1126/science.abi8175. Epub 2022 Apr 29. 10.1126/science.abi8175 PubMed 35482859