pQE80L Hu IL-15-His tag
(Plasmid #183256)

• Purpose: Bacterial expression of human IL-15 with N-terminal His tag
• Depositing Lab: Alexander McLellan
• Publication: McLellan Lab Plasmids 2022 (unpublished)

Backbone

• Vector backbone: pQE-80L
• Vector type: Bacterial Expression

Growth in Bacteria

• Bacterial Resistance(s): Ampicillin, 100 μg/mL
• Growth Temperature: 37°C
• Growth Strain(s): DH5alpha
• Copy number: High Copy

Gene/Insert

• Gene/Insert name: IL-15
• Species: H. sapiens (human)
• Entrez Gene: IL15 (a.k.a. IL-15)
• Tag / Fusion Protein:
  • 6xHis (C terminal on insert)

Cloning Information

• Cloning method: Restriction Enzyme
• 5′ cloning site: unknown (unknown if destroyed)

Resource Information

• Supplemental Documents:
  • pQE80L_Hu IL-15-His tag.gbk

Terms and Licenses

• Academic/Nonprofit Terms:
  • UBMTA
• Industry Terms:
  • Not Available to Industry

Depositor Comments

Expression and inclusion body preparation was carried out essentially as described in Carmenate et al. (2013), except that the 6M guanidine solubilised IL-15 was applied directly to an NTA column at 1 column volume / minute. LPS was removed with 0.1% Triton X114 / TE washes following by further on-column re-folding with TE buffer. Reference: Carmenate T, Pacios A, Enamorado M, Moreno E, Garcia-Martinez K, Fuente D, and Leon K. Human IL-2 mutein with higher antitumor efficacy than wild type IL-2. J. Immunol. 2013; 190:6230-6238. doi.org/10.4049/jimmunol.1201895.