pX4
(Plasmid #183903)

• Purpose: donor plasmid for CRISPR Cas9 knockin near the Aedes aegypti m / M locus
• Depositing Lab: Eric Marois
• Publication: Lutrat et al Commun Biol. 2023 Jun 16;6(1):646. doi: 10.1038/s42003-023-05030-7.

Backbone

• Vector backbone: pENTR R4 ATCC LacZGCTT (Addgene #173668)
• Backbone size w/o insert (bp): 2500
• Total vector size (bp): 6785
• Vector type: Insect Expression

Growth in Bacteria

• Bacterial Resistance(s): Kanamycin, 50 μg/mL
• Growth Temperature: 37°C
• Growth Strain(s): DH5alpha
• Copy number: High Copy

Gene/Insert

• Gene/Insert name: GFP
• Alt name: 3xP3-GFP-DmTub56Dterminator cassette flanked by Ae. aegypti genomic sequences
• Species: Synthetic
• Promoter: Aedes aegypti PUb

Cloning Information

• Cloning method: Restriction Enzyme
• 5′ cloning site: BsaI (Golden Gate Cloning) (destroyed during cloning)
• 3′ cloning site: BsaI (Golden Gate Cloning) (destroyed during cloning)
• 5′ sequencing primer: M13F
• 3′ sequencing primer: M13R

Resource Information

• Supplemental Documents:
  • pX4.gb

Terms and Licenses

• Academic/Nonprofit Terms:
  • UBMTA
• Industry Terms:
  • Not Available to Industry

Depositor Comments

Donor construct designed to insert GFP near the m/M locus of Aedes aegypti using the CRISPR/Cas9 system.

Please visit https://doi.org/10.1101/2022.03.11.483912 for bioRxiv preprint.