LIPS:SDC101a-A08:C34411
(Plasmid #183978)

• Purpose: Baculovirus expression for structure determination. May not contain entire coding region of gene.
• Depositing Lab: Cheryl Arrowsmith
• Publication: SGC Structures-2021-Q3 and Q4 (unpublished)

Backbone

• Vector backbone: pAB- LIC C
• Backbone manufacturer: Plasmid #62288
• Vector type: Baculovirus Expresssion

Growth in Bacteria

• Bacterial Resistance(s): Ampicillin, 100 μg/mL
• Growth Temperature: 37°C
• Growth Strain(s): DH5alpha
• Copy number: High Copy

Gene/Insert

• Gene/Insert name: LIPS
• Alt name: 2OXE
• Species: H. sapiens (human)
• Insert Size (bp): 1356
• Promoter: Polyhedrin
• Tags / Fusion Proteins:
  • N-terminal Honeybee melittin signal:MKFLVNVALVFMVVYISYIYAAA (N terminal on insert)
  • C-terminal 8xHis tag:EFVEHHHHHHHH (C terminal on insert)

Cloning Information

• Cloning method: Ligation Independent Cloning
• 5′ sequencing primer: phF:AGACGCACAAACTAATATCACAAACTGGA
• 3′ sequencing primer: mR:CGTGTCGGGTTTAACATTACGGAT

Terms and Licenses

• Academic/Nonprofit Terms:
  • UBMTA
• Industry Terms:
  • Not Available to Industry

Depositor Comments

SGC Clone Sample ID: LIPS:SDC101a-A08:C34411. PDB link: https://www.rcsb.org/structure/2OXE