pcDNA T7-Gnat1 Mut-3UTR
(Plasmid #184027)

• Purpose: Expresses mouse Gnat1 protein with N-terminal T7 tag from cDNA that contains the mutant 3'-UTR where the TAG binding sites for MSI1 are mutated to TGA
• Depositing Lab: Peter Stoilov
• Publication: Matalkah et al Commun Biol. 2022 Sep 24;5(1):1011. doi: 10.1038/s42003-022-03990-w.

Backbone

• Vector backbone: pcDNA3.1(+)
• Backbone manufacturer: Invitrogen
• Vector type: Mammalian Expression

Growth in Bacteria

• Bacterial Resistance(s): Ampicillin, 100 μg/mL
• Growth Temperature: 37°C
• Growth Strain(s): DH5alpha
• Copy number: High Copy

Gene/Insert

• Gene/Insert name: Gnat1
• Species: M. musculus (mouse)
• Mutation: TAG sites in the 3'-UTR are mutated to TGA
• Entrez Gene: Gnat1 (a.k.a. Gnat-1, Ird1, Ird2, Tralpha, irdc, irdr, transducin)
• Promoter: CMV
• Tag / Fusion Protein:
  • T7 (N terminal on insert)

Cloning Information

• Cloning method: Gibson Cloning
• 5′ sequencing primer: T7
• 3′ sequencing primer: pcDNA3.1/BGH reverse priming site

Resource Information

• A portion of this plasmid was derived from a plasmid made by: Derived from MGC clone ID 6490798

Terms and Licenses

• Academic/Nonprofit Terms:
  • UBMTA
• Industry Terms:
  • Not Available to Industry

Depositor Comments

5' Cloning Site: NheI (destroyed during cloning), 3' Cloning Site: XhoI (destroyed during cloning).

Please visit https://www.biorxiv.org/content/10.1101/2022.03.09.483688v1 for bioRxiv preprint.