PurposeExpresses myc-USP10 construct in mammalian cells
|Item||Catalog #||Description||Quantity||Price (USD)|
|Plasmid||184034||Standard format: Plasmid sent in bacteria as agar stab||1||$85|
This material is available to academics and nonprofits only.
- Backbone size w/o insert (bp) 4006
- Total vector size (bp) 7339
Vector typeMammalian Expression
Growth in Bacteria
Bacterial Resistance(s)Ampicillin, 100 μg/mL
SpeciesH. sapiens (human)
- Promoter CMVf
/ Fusion Protein
- myc (N terminal on insert)
- Cloning method Gibson Cloning
- 5′ sequencing primer CGCAAATGGGCGGTAGGCGTG (Common Sequencing Primers)
Terms and Licenses
- Not Available to Industry
- Zeocin® is an InvivoGen trademark.
These plasmids were created by your colleagues. Please acknowledge the Principal Investigator, cite the article in which the plasmids were described, and include Addgene in the Materials and Methods of your future publications.
For your Materials & Methods section:pCI-myc-USP10 was a gift from Juan Bonifacino (Addgene plasmid # 184034 ; http://n2t.net/addgene:184034 ; RRID:Addgene_184034)
For your References section:The ubiquitin isopeptidase USP10 deubiquitinates LC3B to increase LC3B levels and autophagic activity. Jia R, Bonifacino JS. J Biol Chem. 2021 Jan-Jun;296:100405. doi: 10.1016/j.jbc.2021.100405. Epub 2021 Feb 10. 10.1016/j.jbc.2021.100405 PubMed 33577797