pYMA3_SidK_GFP
(Plasmid
#184276)
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PurposeLegionella pneumophila SidK (1-278) - GFP
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Depositing Lab
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Sequence Information
Ordering
| Item | Catalog # | Description | Quantity | Price (USD) | |
|---|---|---|---|---|---|
| Plasmid | 184276 | Standard format: Plasmid sent in bacteria as agar stab | 1 | $85 | |
This material is available to academics and nonprofits only.
Backbone
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Vector backbonepEGFP-N1
- Total vector size (bp) 5544
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Vector typeMammalian Expression
Growth in Bacteria
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Bacterial Resistance(s)Kanamycin, 50 μg/mL
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Growth Temperature37°C
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Growth Strain(s)DH5alpha
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Copy numberUnknown
Gene/Insert
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Gene/Insert nameSidK (1-278)
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SpeciesLegionella pneumophila
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Insert Size (bp)834
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Entrez GenesidK (a.k.a. A9E85_RS04790, A9E85_04790)
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Tag
/ Fusion Protein
- GFP (C terminal on insert)
Terms and Licenses
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Academic/Nonprofit Terms
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Industry Terms
- Not Available to Industry
Trademarks:
- Zeocin® is an InvivoGen trademark.
How to cite this plasmid
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These plasmids were created by your colleagues. Please acknowledge the Principal Investigator, cite the article in which the plasmids were described, and include Addgene in the Materials and Methods of your future publications.
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For your Materials & Methods section:
pYMA3_SidK_GFP was a gift from John Rubinstein (Addgene plasmid # 184276 ; http://n2t.net/addgene:184276 ; RRID:Addgene_184276) -
For your References section:
Detection and quantification of the vacuolar H+ATPase using the Legionella effector protein SidK. Maxson ME, Abbas YM, Wu JZ, Plumb JD, Grinstein S, Rubinstein JL. J Cell Biol. 2022 Mar 7;221(3). pii: 212963. doi: 10.1083/jcb.202107174. Epub 2022 Jan 13. 10.1083/jcb.202107174 PubMed 35024770
