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pYMA3_SidK_GFP
(Plasmid #184276)

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This material is available to academics and nonprofits only.
Item Catalog # Description Quantity Price (USD)
Plasmid 184276 Standard format: Plasmid sent in bacteria as agar stab 1 $85
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Backbone

  • Vector backbone
    pEGFP-N1
  • Total vector size (bp) 5544
  • Vector type
    Mammalian Expression

Growth in Bacteria

  • Bacterial Resistance(s)
    Kanamycin, 50 μg/mL
  • Growth Temperature
    37°C
  • Growth Strain(s)
    DH5alpha
  • Copy number
    Unknown

Gene/Insert

  • Gene/Insert name
    SidK (1-278)
  • Species
    Legionella pneumophila
  • Insert Size (bp)
    834
  • Entrez Gene
    sidK (a.k.a. A9E85_RS04790, A9E85_04790)
  • Tag / Fusion Protein
    • GFP (C terminal on insert)

Terms and Licenses

  • Academic/Nonprofit Terms
  • Industry Terms
    • Not Available to Industry
Trademarks:
  • Zeocin® is an InvivoGen trademark.
How to cite this plasmid ( Back to top)

These plasmids were created by your colleagues. Please acknowledge the Principal Investigator, cite the article in which the plasmids were described, and include Addgene in the Materials and Methods of your future publications.

  • For your Materials & Methods section:

    pYMA3_SidK_GFP was a gift from John Rubinstein (Addgene plasmid # 184276 ; http://n2t.net/addgene:184276 ; RRID:Addgene_184276)

  • For your References section:

    Detection and quantification of the vacuolar H+ATPase using the Legionella effector protein SidK. Maxson ME, Abbas YM, Wu JZ, Plumb JD, Grinstein S, Rubinstein JL. J Cell Biol. 2022 Mar 7;221(3). pii: 212963. doi: 10.1083/jcb.202107174. Epub 2022 Jan 13. 10.1083/jcb.202107174 PubMed 35024770
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