ACE2-IgFc probe
(Plasmid
#184787)
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PurposeExpression of His-tagged, soluble ACE2-IgFc microbody
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Depositing Lab
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Sequence Information
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Sequences (1) — Accept Affinity Reagent Sequence Policy
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Ordering
| Item | Catalog # | Description | Quantity | Price (USD) | |
|---|---|---|---|---|---|
| Plasmid | 184787 | Standard format: Plasmid sent in bacteria as agar stab | 1 | $85 | |
This material is available to academics and nonprofits only.
Backbone
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Vector backbonepWPI-IRES-Puro-Ak
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Vector typeMammalian Expression
Growth in Bacteria
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Bacterial Resistance(s)Ampicillin, 100 μg/mL
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Growth Temperature37°C
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Growth Strain(s)DH5alpha
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Copy numberLow Copy
Gene/Insert
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Gene/Insert namehACE2-IgFc
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SpeciesSynthetic
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Entrez GeneACE2 (a.k.a. ACEH)
- Promoter EF1-alpha
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Tag
/ Fusion Protein
- 6xHis Tag (C terminal on insert)
Cloning Information
- Cloning method Restriction Enzyme
- 5′ cloning site unknown (unknown if destroyed)
- 3′ cloning site unknown (unknown if destroyed)
- 5′ sequencing primer unknown (Common Sequencing Primers)
Terms and Licenses
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Academic/Nonprofit Terms
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Industry Terms
- Not Available to Industry
Trademarks:
- Zeocin® is an InvivoGen trademark.
How to cite this plasmid
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These plasmids were created by your colleagues. Please acknowledge the Principal Investigator, cite the article in which the plasmids were described, and include Addgene in the Materials and Methods of your future publications.
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For your Materials & Methods section:
ACE2-IgFc probe was a gift from Ajit Chande (Addgene plasmid # 184787 ; http://n2t.net/addgene:184787 ; RRID:Addgene_184787) -
For your References section:
SARS CoV-2 Nucleoprotein Enhances the Infectivity of Lentiviral Spike Particles. Mishra T, Sreepadmanabh M, Ramdas P, Sahu AK, Kumar A, Chande A. Front Cell Infect Microbiol. 2021 Apr 23;11:663688. doi: 10.3389/fcimb.2021.663688. eCollection 2021. 10.3389/fcimb.2021.663688 PubMed 33968806
