pJT396 PB ITR and Cp36 attD pEF Puro-2a-tagBFP2 donor
(Plasmid
#184952)
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PurposeDonor for both PiggyBac and Cp36 Recombinase
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Depositing Labs
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Sequence Information
Ordering
| Item | Catalog # | Description | Quantity | Price (USD) | |
|---|---|---|---|---|---|
| Plasmid | 184952 | Standard format: Plasmid sent in bacteria as agar stab | 1 | $85 | |
This material is available to academics and nonprofits only.
Backbone
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Vector backbonen/a
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Vector typeMammalian Expression
Growth in Bacteria
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Bacterial Resistance(s)Ampicillin, 100 μg/mL
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Growth Temperature37°C
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Growth Strain(s)NEB Stable
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Copy numberHigh Copy
Gene/Insert
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Gene/Insert namePuroR, P2A, mTagBFP2
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SpeciesSynthetic
Cloning Information
- Cloning method Restriction Enzyme
- 5′ cloning site unknown (unknown if destroyed)
Resource Information
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Supplemental Documents
Terms and Licenses
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Academic/Nonprofit Terms
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Industry Terms
- Not Available to Industry
Trademarks:
- Zeocin® is an InvivoGen trademark.
Depositor Comments
Donor DNA that is compatible with both PiggyBac transposase and with Cp36 recombinase
How to cite this plasmid
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These plasmids were created by your colleagues. Please acknowledge the Principal Investigator, cite the article in which the plasmids were described, and include Addgene in the Materials and Methods of your future publications.
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For your Materials & Methods section:
pJT396 PB ITR and Cp36 attD pEF Puro-2a-tagBFP2 donor was a gift from Michael Bassik & Ami Bhatt & Lacra Bintu & Patrick Hsu (Addgene plasmid # 184952 ; http://n2t.net/addgene:184952 ; RRID:Addgene_184952) -
For your References section:
Systematic discovery of recombinases for efficient integration of large DNA sequences into the human genome. Durrant MG, Fanton A, Tycko J, Hinks M, Chandrasekaran SS, Perry NT, Schaepe J, Du PP, Lotfy P, Bassik MC, Bintu L, Bhatt AS, Hsu PD. Nat Biotechnol. 2022 Oct 10. pii: 10.1038/s41587-022-01494-w. doi: 10.1038/s41587-022-01494-w. 10.1038/s41587-022-01494-w PubMed 36217031
