pEGFP-C1-G2BRgp78
(Plasmid #185344)

• Purpose: Expression of soluble G2BR, amino acids 575-600 of human gp78
• Depositing Lab: Allan Weissman
• Publication: Smith et al PLoS Biol. 2021 Dec 8;19(12):e3001474. doi: 10.1371/journal.pbio.3001474. eCollection 2021 Dec.

Backbone

• Vector backbone: pEGFP-C1
• Vector type: Mammalian Expression

Growth in Bacteria

• Bacterial Resistance(s): Kanamycin, 50 μg/mL
• Growth Temperature: 37°C
• Growth Strain(s): DH5alpha
• Copy number: High Copy

Gene/Insert

• Gene/Insert name: gp78 aa 574-600 (G2BR)
• Species: H. sapiens (human)
• Mutation: aa 575-600 only
• Entrez Gene: AMFR (a.k.a. GP78, RNF45)
• Tag / Fusion Protein:
  • EGFP (N terminal on backbone)

Cloning Information

• Cloning method: Restriction Enzyme
• 5′ cloning site: Xba1 (unknown if destroyed)
• 3′ cloning site: EcoR1 (unknown if destroyed)
• 5′ sequencing primer: '

Terms and Licenses

• Academic/Nonprofit Terms:
  • UBMTA
• Industry Terms:
  • Not Available to Industry