pGEX-6P-1-gp78 RING
(Plasmid #185353)

• Purpose: RING domain-containing GST fusion protein for ubiquitination assays.
• Depositing Lab: Allan Weissman
• Publication: Smith et al PLoS Biol. 2021 Dec 8;19(12):e3001474. doi: 10.1371/journal.pbio.3001474. eCollection 2021 Dec.

Backbone

• Vector backbone: pGEX-6P-1
• Vector type: Bacterial Expression

Growth in Bacteria

• Bacterial Resistance(s): Ampicillin, 100 μg/mL
• Growth Temperature: 37°C
• Growth Strain(s): DH5alpha
• Copy number: High Copy

Gene/Insert

• Gene/Insert name: gp78 cytoplasmic domain
• Species: H. sapiens (human)
• Mutation: Stop codon after aa 393
• Entrez Gene: AMFR (a.k.a. GP78, RNF45)
• Tag / Fusion Protein:
  • GST (N terminal on backbone)

Cloning Information

• Cloning method: Restriction Enzyme
• 5′ cloning site: BamHI (not destroyed)
• 3′ cloning site: SalI (not destroyed)
• 5′ sequencing primer: -

Terms and Licenses

• Academic/Nonprofit Terms:
  • UBMTA
• Industry Terms:
  • Not Available to Industry

Depositor Comments

Plasmid contains sequence encoding the entire C-terminus of gp78. Stop codon placed following the RING domain to limit expression to GST-RING domain fusion.