Skip to main content
This website uses cookies to ensure you get the best experience. By continuing to use this site, you agree to the use of cookies.

Please note: Your browser does not support the features used on Addgene's website. You may not be able to create an account or request plasmids through this website until you upgrade your browser. Learn more

Please note: Your browser does not fully support some of the features used on Addgene's website. If you run into any problems registering, depositing, or ordering please contact us at [email protected] Learn more

pCS2+ Zf cdh1-deltaCyot-GFP
(Plasmid #185412)


Item Catalog # Description Quantity Price (USD)
Plasmid 185412 Standard format: Plasmid sent in bacteria as agar stab 1 $75

This material is available to academics and nonprofits only.


  • Vector backbone
  • Backbone size w/o insert (bp) 4095
  • Total vector size (bp) 6996
  • Vector type
    Mammalian Expression ; In vitro synthesis of mRNA

Growth in Bacteria

  • Bacterial Resistance(s)
    Ampicillin, 100 μg/mL
  • Growth Temperature
  • Growth Strain(s)
  • Copy number
    High Copy


  • Gene/Insert name
  • Alt name
  • Species
    D. rerio (zebrafish)
  • Insert Size (bp)
  • Mutation
    Cytoplasmic domain deleted
  • GenBank ID
  • Entrez Gene
    cdh1 (a.k.a. E-cadherin)
  • Tag / Fusion Protein
    • eGFP (C terminal on backbone)

Cloning Information

Resource Information

  • A portion of this plasmid was derived from a plasmid made by
    Dr E. Raz, University of Münster

Terms and Licenses

  • Zeocin® is an InvivoGen trademark.

Depositor Comments

How to cite this plasmid ( Back to top)

These plasmids were created by your colleagues. Please acknowledge the Principal Investigator, cite the article in which the plasmids were described, and include Addgene in the Materials and Methods of your future publications.

  • For your Materials & Methods section:

    pCS2+ Zf cdh1-deltaCyot-GFP was a gift from Nicolas David (Addgene plasmid # 185412 ; ; RRID:Addgene_185412)
  • For your References section:

    Guidance by followers ensures long-range coordination of cell migration through alpha-catenin mechanoperception. Boutillon A, Escot S, Elouin A, Jahn D, Gonzalez-Tirado S, Starruss J, Brusch L, David NB. Dev Cell. 2022 May 17. pii: S1534-5807(22)00329-X. doi: 10.1016/j.devcel.2022.05.001. 10.1016/j.devcel.2022.05.001 PubMed 35613615