LDB34
(Plasmid #185431)

• Purpose: Yeast expression of Nup1 C-terminal truncation (nup1-21 allele)
• Depositing Labs: Ken Belanger, Laura Davis
• Publication: Bogerd et al J Cell Biol. 1994 Oct . 127(2):319-32.

Backbone

• Vector backbone: pRS314
• Vector type: Yeast Expression
• Selectable markers: TRP1

Growth in Bacteria

• Bacterial Resistance(s): Ampicillin, 100 μg/mL
• Growth Temperature: 37°C
• Growth Strain(s): DH5alpha
• Copy number: High Copy

Gene/Insert

• Gene/Insert name: NUP1
• Species: S. cerevisiae (budding yeast)
• Mutation: Truncation at amino acid 1041 from the C-term of Nup1

Cloning Information

• Cloning method: Restriction Enzyme
• 5′ cloning site: unknown (unknown if destroyed)

Terms and Licenses

• Academic/Nonprofit Terms:
  • UBMTA
• Industry Terms:
  • Not Available to Industry

Depositor Comments

Please note: Plasmid contains an additional 22 amino acids (LKDIYSYIPSTYTLHMKLLEKF) on the C-terminus of Nup1 compared to the NCBI reference sequence NP_014741.1. It is not known how this affect the plasmid's function.