pAAV-hSyn-GFP-IRES-hSNCA (S129A)
(Plasmid #185718)

• Purpose: AAV expression of GFP and human α-Synuclein with S129A mutation from hSyn promoter
• Depositing Lab: Jun Ding
• Publication: Albarran et al Nat Neurosci. 2023 Jun;26(6):997-1007. doi: 10.1038/s41593-023-01345-0. Epub 2023 May 29.

Backbone

• Vector backbone: pAAV-hSyn-EGFP
• Backbone size w/o insert (bp): 4874
• Total vector size (bp): 6359
• Vector type: AAV

Growth in Bacteria

• Bacterial Resistance(s): Ampicillin, 100 μg/mL
• Growth Temperature: 30°C
• Growth Strain(s): NEB Stable
• Copy number: Low Copy

Gene/Insert

• Gene/Insert name: synuclein, alpha
• Alt name: SNCA
• Species: H. sapiens (human)
• Insert Size (bp): 423
• Mutation: Changed Ser 129 to Ala
• GenBank ID: NM_000345
• Entrez Gene: SNCA (a.k.a. NACP, PARK1, PARK4, PD1)
• Promoter: hSyn

Cloning Information

• Cloning method: Restriction Enzyme
• 5′ cloning site: SalI (not destroyed)
• 3′ cloning site: NheI (not destroyed)
• 5′ sequencing primer: EGFP-C
• 3′ sequencing primer: WPRE-R

Resource Information

• Supplemental Documents:
  • C.56-pAAV-Syn-GFP-IRES-hSNCA (S129A).gbk
• A portion of this plasmid was derived from a plasmid made by: Thomas C. Südhof, Stanford

Terms and Licenses

• Academic/Nonprofit Terms:
  • UBMTA
• Industry Terms:
  • Not Available to Industry

Depositor Comments

Please visit https://www.biorxiv.org/content/10.1101/2021.10.04.462870v1 for bioRxiv preprint.