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Addgene

pMCSG28-SH3-588
(Plasmid #185920)

Ordering

This material is available to academics and nonprofits only.
Item Catalog # Description Quantity Price (USD)
Plasmid 185920 Standard format: Plasmid sent in bacteria as agar stab 1 $85

Backbone

  • Vector backbone
    pMCSG28
  • Backbone size w/o insert (bp) 5000
  • Total vector size (bp) 6000
  • Modifications to backbone
    Insertion between RBS and TEV/6x HIS site
  • Vector type
    Bacterial Expression

Growth in Bacteria

  • Bacterial Resistance(s)
    Ampicillin, 100 μg/mL
  • Growth Temperature
    37°C
  • Growth Strain(s)
    DH5alpha
  • Copy number
    Unknown

Gene/Insert

  • Gene/Insert name
    SH3-588
  • Species
    Synthetic
  • Insert Size (bp)
    900
  • Mutation
    Designed enzyme with novel interface between humanized rat COMT and the human Fyn SH3 domain (numerous mutations)
  • Promoter T7
  • Tag / Fusion Protein
    • 6x HIS tag (C terminal on insert)

Cloning Information

Terms and Licenses

  • Academic/Nonprofit Terms
  • Industry Terms
    • Not Available to Industry
Trademarks:
  • Zeocin® is an InvivoGen trademark.
How to cite this plasmid ( Back to top)

These plasmids were created by your colleagues. Please acknowledge the Principal Investigator, cite the article in which the plasmids were described, and include Addgene in the Materials and Methods of your future publications.

  • For your Materials & Methods section:

    pMCSG28-SH3-588 was a gift from Brian Kuhlman (Addgene plasmid # 185920 ; http://n2t.net/addgene:185920 ; RRID:Addgene_185920)
  • For your References section:

    Designer installation of a substrate recruitment domain to tailor enzyme specificity. Park R, Ongpipattanakul C, Nair SK, Bowers AA, Kuhlman B. Nat Chem Biol. 2023 Apr;19(4):460-467. doi: 10.1038/s41589-022-01206-0. Epub 2022 Dec 12. 10.1038/s41589-022-01206-0 PubMed 36509904