pMCSG28-SH3-588
(Plasmid
#185920)
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PurposeExpresses SH3-588, a designed poly-proline peptide recruiting catechol O-methyltransferase
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Depositing Lab
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Sequence Information
Ordering
Item | Catalog # | Description | Quantity | Price (USD) | |
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Plasmid | 185920 | Standard format: Plasmid sent in bacteria as agar stab | 1 | $85 |
Backbone
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Vector backbonepMCSG28
- Backbone size w/o insert (bp) 5000
- Total vector size (bp) 6000
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Modifications to backboneInsertion between RBS and TEV/6x HIS site
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Vector typeBacterial Expression
Growth in Bacteria
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Bacterial Resistance(s)Ampicillin, 100 μg/mL
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Growth Temperature37°C
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Growth Strain(s)DH5alpha
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Copy numberUnknown
Gene/Insert
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Gene/Insert nameSH3-588
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SpeciesSynthetic
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Insert Size (bp)900
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MutationDesigned enzyme with novel interface between humanized rat COMT and the human Fyn SH3 domain (numerous mutations)
- Promoter T7
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Tag
/ Fusion Protein
- 6x HIS tag (C terminal on insert)
Cloning Information
- Cloning method Gibson Cloning
- 5′ sequencing primer T7 promoter
- 3′ sequencing primer T7 terminator (Common Sequencing Primers)
Terms and Licenses
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Academic/Nonprofit Terms
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Industry Terms
- Not Available to Industry
Trademarks:
- Zeocin® is an InvivoGen trademark.
These plasmids were created by your colleagues. Please acknowledge the Principal Investigator, cite the article in which the plasmids were described, and include Addgene in the Materials and Methods of your future publications.
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For your Materials & Methods section:
pMCSG28-SH3-588 was a gift from Brian Kuhlman (Addgene plasmid # 185920 ; http://n2t.net/addgene:185920 ; RRID:Addgene_185920) -
For your References section:
Designer installation of a substrate recruitment domain to tailor enzyme specificity. Park R, Ongpipattanakul C, Nair SK, Bowers AA, Kuhlman B. Nat Chem Biol. 2023 Apr;19(4):460-467. doi: 10.1038/s41589-022-01206-0. Epub 2022 Dec 12. 10.1038/s41589-022-01206-0 PubMed 36509904