pB6-Rosa26-cre_dre_reporter_1_2
(Plasmid #185966)

• Purpose: B6J Rosa26-cre dre reporter 1.2 allele targeting vector, low copy number
• Depositing Lab: Mario Capecchi
• Publication: Miscellaneous gene targeting vector plasmids (unpublished)

Backbone

• Vector backbone: Custom
• Backbone manufacturer: Hyung-song Nam, Capecchi laboratory
• Vector type: Mammalian Expression, Mouse Targeting, Cre/Lox ; Dre/Rox
• Selectable markers: Neomycin (select with G418)

Growth in Bacteria

• Bacterial Resistance(s): Ampicillin, 100 μg/mL
• Growth Temperature: 30°C
• Growth Strain(s): DH10B
• Copy number: Low Copy

Gene/Insert

• Gene/Insert name: sfGFP, tdTomato
• Species: Synthetic

Resource Information

• Supplemental Documents:
  • pROSA26-DTA-cre dre reporter 1.2.gb
• A portion of this plasmid was derived from a plasmid made by: FNF is from plasmid #22687. CAG promoter is from pCAGGS obtained from BCCM/LMBP. sfGFP is from Sandia Biotech. WPRE is from Miguel Ramalho-Santos. bGH pA is from Neal Copeland. SV40 pA is from Clontech. EMCV IRES is from Peter Mombaerts. tdTomato is from Roger Tsien.

Terms and Licenses

• Academic/Nonprofit Terms:
  • UBMTA
  • Takara Bio Limited Use Label License (formerly Clontech)
• Industry Terms:
  • Not Available to Industry

Depositor Comments

cre → sfGFP expression. dre → tdTomato expression.

Expression of sfGFP and tdTomato are independent of each other. "OR."

Actual mouse was not generated.

The EMCV IRES fragment from Dr. Peter Mombaerts is the same EMCV IRES used in the Id1-IRES-creERT2 knock-in mouse line (http://www.informatics.jax.org/allele/MGI:4366863).