pNB_EA_R1CiM_4EGFP_R3EGFPi2
(Plasmid
#185968)
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PurposeMolecular IMPLY gate, encodes mutant Ci protein from Plteto-1 promoter, and EGFP form PR and PlTataa promoter. Must be expressed in E.coli DH5alphaz1 for experimental characterization.
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Depositing Lab
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Sequence Information
Ordering
| Item | Catalog # | Description | Quantity | Price (USD) | |
|---|---|---|---|---|---|
| Plasmid | 185968 | Standard format: Plasmid sent in bacteria as agar stab | 1 | $85 | |
This material is available to academics and nonprofits only.
Backbone
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Vector backbonePz
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Backbone manufacturerexpressys.
- Backbone size w/o insert (bp) 1856
- Total vector size (bp) 5059
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Modifications to backboneINSERTED molecular IMPLY GATE
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Vector typeSynthetic Biology
Growth in Bacteria
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Bacterial Resistance(s)Ampicillin, 100 μg/mL
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Growth Temperature37°C
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Growth Strain(s)DH5alpha
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Copy numberHigh Copy
Gene/Insert
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Gene/Insert nameMolecular IMPLY gate
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SpeciesSynthetic
Cloning Information
- Cloning method Restriction Enzyme
- 5′ cloning site AatII (not destroyed)
- 3′ cloning site PciI (not destroyed)
- 5′ sequencing primer TACTGGGACGAAGACGAACA
- 3′ sequencing primer GTTGTTTTGGAGCACGGAAC (Common Sequencing Primers)
Resource Information
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Supplemental Documents
Terms and Licenses
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Academic/Nonprofit Terms
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Industry Terms
- Not Available to Industry
Trademarks:
- Zeocin® is an InvivoGen trademark.
How to cite this plasmid
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These plasmids were created by your colleagues. Please acknowledge the Principal Investigator, cite the article in which the plasmids were described, and include Addgene in the Materials and Methods of your future publications.
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For your Materials & Methods section:
pNB_EA_R1CiM_4EGFP_R3EGFPi2 was a gift from Sangram Bagh (Addgene plasmid # 185968 ; http://n2t.net/addgene:185968 ; RRID:Addgene_185968) -
For your References section:
Processing two environmental chemical signals with a synthetic genetic IMPLY gate, a 2-input-2-output integrated logic circuit, and a process pipeline to optimize its systems chemistry in Escherichia coli. Mukhopadhyay S, Sarkar K, Srivastava R, Pal A, Bagh S. Biotechnol Bioeng. 2020 May;117(5):1502-1512. doi: 10.1002/bit.27286. Epub 2020 Feb 7. 10.1002/bit.27286 PubMed 31981217
Map uploaded by the depositor.
