T_antitdTomatosynsrRNA_UA
(Plasmid #185975)

• Purpose: Expresses antitdTomatosynsrRNA from PlTetO-1 promoter in E.coli Dh5alpha cell. For experiment use Dh5alphaz1.
• Depositing Lab: Sangram Bagh
• Publication: Mukhopadhyay et al Biosens Bioelectron. 2020 Nov 1;167:112462. doi: 10.1016/j.bios.2020.112462. Epub 2020 Aug 1.

Backbone

• Vector backbone: Pz
• Backbone manufacturer: expressys.
• Backbone size w/o insert (bp): 2112
• Total vector size (bp): 2548
• Modifications to backbone: Insertion of PlTetO-1antitdTomatosynsrRNA
• Vector type: Synthetic Biology

Growth in Bacteria

• Bacterial Resistance(s): Ampicillin, 100 μg/mL
• Growth Temperature: 37°C
• Growth Strain(s): DH5alpha
• Copy number: High Copy

Gene/Insert

• Gene/Insert name: PlTetO-1 AntitdTomatosynsrRNA
• Species: Synthetic
• Insert Size (bp): 436
• Promoter: PlTetO-1

Cloning Information

• Cloning method: Restriction Enzyme
• 5′ cloning site: AatII (not destroyed)
• 3′ cloning site: AvrII (not destroyed)
• 5′ sequencing primer: GAGGCCCTTTCGTCTTCAC

Resource Information

• Supplemental Documents:
  • T_antitdTomatosynsrRNA_UA.gb

Terms and Licenses

• Academic/Nonprofit Terms:
  • UBMTA
• Industry Terms:
  • Not Available to Industry

Depositor Comments

Please visit https://www.biorxiv.org/content/10.1101/2020.01.26.920629v1 for bioRxiv preprint.