Nephrin
(Plasmid #186134)

• Purpose: For expression of recombinant nephrin in bacteria cells
• Depositing Lab: Michael Rosen
• Publication: Case et al Elife. 2022 Jan 20;11. pii: 72588. doi: 10.7554/eLife.72588.

Backbone

• Vector backbone: pMal-RTH
• Backbone size w/o insert (bp): 6600
• Vector type: Bacterial Expression

Growth in Bacteria

• Bacterial Resistance(s): Ampicillin, 100 μg/mL
• Growth Temperature: 37°C
• Growth Strain(s): DH5alpha
• Copy number: Low Copy

Gene/Insert

• Gene/Insert name: NPHS1
• Species: H. sapiens (human)
• Insert Size (bp): 219
• Mutation: Amino acid residues 1174-1223, with mutations: Y1183F, Y1210F
• Entrez Gene: NPHS1 (a.k.a. CNF, NPHN, nephrin)
• Promoter: Lac
• Tags / Fusion Proteins:
  • MBP (N terminal on insert)
  • His6 (C terminal on insert)

Cloning Information

• Cloning method: Restriction Enzyme
• 5′ cloning site: NdeI (not destroyed)
• 3′ cloning site: BamHI (not destroyed)
• 5′ sequencing primer: pMAL-Fwd
• 3′ sequencing primer: pMAL-Rev

Terms and Licenses

• Academic/Nonprofit Terms:
  • UBMTA
• Industry Terms:
  • Not Available to Industry