pKI-cjFOXL2-coemiRFP670-PGK-Puro
(Plasmid
#186168)
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PurposeKnock-in gene targeting in marmoset cells at FOXL2 locus
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Depositing Lab
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Depositing OrganizationKeio University
Located in Japan -
Sequence Information
Ordering
| Item | Catalog # | Description | Quantity | Price (USD) | |
|---|---|---|---|---|---|
| Plasmid | 186168 | Standard format: Plasmid sent in bacteria as agar stab | 1 | $94 | |
| DNA | 186168-D50 | 50 μg of DNA in Tris buffer | $495 | ||
| DNA | 186168-D100 | 100 μg of DNA in Tris buffer | $585 | ||
Backbone
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Vector backbonepCR-Blunt II-TOPO
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Backbone manufacturerThermo Fisher Scientific
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Vector typeMarmoset targeting
Growth in Bacteria
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Bacterial Resistance(s)Ampicillin, 100 μg/mL
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Growth Temperature37°C
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Growth Strain(s)NEB Stable
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Copy numberHigh Copy
Gene/Insert
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Gene/Insert nameemiRFP670
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SpeciesRhodopseudomonas palustris
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Mutationhuman codon-optimized
Cloning Information
- Cloning method Restriction Enzyme
- 5′ cloning site unknown (unknown if destroyed)
Resource Information
Terms and Licenses
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Academic/Nonprofit Terms
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Industry Terms
- Not Available to Industry
Trademarks:
- Zeocin® is an InvivoGen trademark.
DNA (Catalog # 186168-D50)
Purpose
Ready-to-use, high-purity DNA prep. DNA aliquots are suitable for use in mammalian cell transfections and other molecular biology applications.
Delivery
- Amount 50 μg
- Concentration 1 μg/μL
- Pricing $495 USD
- Storage 4 ℃ (short-term) or -20 ℃ (long-term)
Terms and Licenses
-
Academic/Nonprofit Terms
-
Industry Terms
- Not Available to Industry
Quality Control
Every DNA prep is verified by next-generation sequencing, confirming plasmid identity, sequence integrity, and absence of DNA contaminants. Concentration is confirmed to be within ±2.5% of the stated concentration.
Our production process has been validated to consistently yield endotoxin levels below 1.0 EU/μg. Individual lots are not tested for endotoxin.
DNA (Catalog # 186168-D100)
Purpose
Ready-to-use, high-purity DNA prep. DNA aliquots are suitable for use in mammalian cell transfections and other molecular biology applications.
Delivery
- Amount 100 μg
- Concentration 1 μg/μL
- Pricing $585 USD
- Storage 4 ℃ (short-term) or -20 ℃ (long-term)
Terms and Licenses
-
Academic/Nonprofit Terms
-
Industry Terms
- Not Available to Industry
Quality Control
Every DNA prep is verified by next-generation sequencing, confirming plasmid identity, sequence integrity, and absence of DNA contaminants. Concentration is confirmed to be within ±2.5% of the stated concentration.
Our production process has been validated to consistently yield endotoxin levels below 1.0 EU/μg. Individual lots are not tested for endotoxin.
These plasmids were created by your colleagues. Please acknowledge the Principal Investigator, cite the article in which the plasmids were described, and include Addgene in the Materials and Methods of your future publications.
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For your Materials & Methods section:
pKI-cjFOXL2-coemiRFP670-PGK-Puro was a gift from Sho Yoshimatsu (Addgene plasmid # 186168 ; http://n2t.net/addgene:186168 ; RRID:Addgene_186168) -
For your References section:
A New Horizon in Reproductive Research with Pluripotent Stem Cells: Successful In Vitro Gametogenesis in Rodents, Its Application to Large Animals, and Future In Vitro Reconstitution of Reproductive Organs Such as "Uteroid" and "Oviductoid". Yoshimatsu S, Kisu I, Qian E, Noce T. Biology (Basel). 2022 Jun 29;11(7):987. doi: 10.3390/biology11070987. 10.3390/biology11070987 PubMed 36101367
